The Polymorphism Of Interleukin17G-152A And Its Association With Bronchial Asthma

Objective:T cells subgroup—T help cell(Th17) secretes the interleukin17(IL-17). IL-17A,a member of IL-17family, who plays a critical role in the pathogenesis of allergicinflammation, and also associates with the occurrence and development of bronchialasthma. Although there are many related research on the polymorphism of IL-17G-152A and its association with autoimmune diseases, few research were carried out onthe correlation between the polymorphism of IL-17G-152A and bronchial asthma. Thepurpose of this paper was to detect the expression of the gene of IL-17G-152A inasthmatic patients and to investigate the role of single nucleotide polymorphism ofIL-17G-152A in the pathogenesis of asthma.Methods:It was a case-control study．Peripheral venous blood were obtained from103cases with asthma(asthma group) and48normal subjects(control group)．GenomicDNAs were extracted from blood samples of the study. SNP genotyping—IL-17G-152A（rs2275913）were performed using the Real-Time PCR system. Data wereanalysed using the Statistical Package of the SPSS18.0. Descriptive data were given asmeans±SD（x±s）．Continuous variables were tested by analysis of t-test andcomparisons of the distributions of the allele and genotype frequencies were performedusing the chi-square test. The relation among the allele、genotype and the incidence ofasthma were tested by Logistic regression.Results:1. IL-17G-152A polymorphisms presents in northeast of China. The quality ofthe genotype data was evaluated by the Hardy-Weinberg equilibrium (HWE) proportiontests. The frequencies of allele was45.8%(A) and54.2%(G) in the healthy population,with a genotype AA frequency of20.8%, AG frequency of50%and GG frequency of 29.2%.2. There was no statistic significance in the frequencies of allele A （χ2=0.108,P=0.742＞0.05）and genotype AA（χ2=0.977，P=0.614＞0.05）in asthma group and incontrol group.3. There was no significant difference in the proportions of subjects withabnormal peripheral blood eosinophilic granulocyte、total serum IgE、FEV1/pred%values and FEV1/FVC%values within genotypes(P＞0.05).4. Logistic regression analysis was used to explore the allele and its genotypewith the onset of asthma, P values>0.05, influences on the disease were not significant.Regression model’s contribution to the disease mutation was6%.Conclusions:1. IL-17G-152A polymorphisms presents in northeastern Han Chinesepopulation.2. There was no correlation between the polymorphism of IL-17G-152A and theoccurrence of asthma in northeast of China. We haven’t found that the homozygous Aallele and AA genotyping carriers increased the risk of developing the asthma. In otherwords, rs2275913SNP was not associated with asthma susceptibility.3. The polymorphism of IL-17G-152A was not associated with the proportions ofsubjects with abnormal peripheral blood eosinophilic granulocyte、total serum IgE、FEV1/pred%values and FEV1/FVC%values.