The Relation Between The Expression Of CCR7,L-Selectin, CD44V6, MMP9in Colorectal Carcinoma And Lymphatic Metastatic

Human colorectal carcinoma is one of the most popular human malignant tumor, which is a serious threat to human health and life. In recent years, the incidence of colorectal carcinoma is rising in China. Tumor invasion and metastasis is the main reason that led to the death of patients with colorectal carcinoma. Therefore, in-depth study of the molecular mechanisms of lymph node metastasis has important social and practical significance.This study on those selected lymphatic homing receptors and related factors CCR7, L-selectin, CD44v6, MMP9. Comparative analysis of the relation between the clinicopathological parameters of colorectal carcinoma, and judgment and analysis the relationship of these factors and lymph node metastasis of colorectal carcinoma. In order to provide theoretical guidance for the clinical predictors of colorectal carcinoma metastasis and treatment.In this study immunohistochemistry for CCR7, L-selectin, CD44v6, MMP9expression was on paraffin sections from104colorectal carcinoma,34cases of metastatic tumor tissue and51cases normal intestinal mucosa.Flow Cytometry(FCM) was used to quantify the concentrations of CCR7, L-selectin, CD44v6and MMP9in30colorectal carcinoma,17cases normal intestinal mucosa specimens.SPSS17.0was applied to analyze the experiment results.P≤0.05indicates significant difference;P≤0.01indicates very significant difference.In this study, get the following results:1The result detected by IHC:There was statistic significant difference (P<0.01) among expression of CCR7, L-selectin, CD44v6, MMP9from colorectal carcinoma, metastatic tumor tissue and normal intestinal mucosa. Expression of CCR7, L-selectin, CD44v6, MMP9was related with lymph node metastasis(P<0.05); But they were not related to patients’sex, patients’age, location of the tumor and pathological grading of tumor(P>0.05).2The results detected by FCM:the quantified expression of CCR7, L-selectin, CD44v6, MMP9(FI=1.76±0.12,1.95±0.16,2.06±0.14,2.15±0.18)in colorectal carcinoma was relatively higher than those in normal intestinal mucosa (FI=1.00±0.17,1.00±0.14,1.00±0.16,1.02±0.17, P<0.01).Expression of CCR7, L-selectin, CD44v6, MMP9was related with lymph node metastasis(P<0.01). But it was not related with patients’sex, patients’age, location of the tumor and pathological grading of tumor (P>0.05).3The correlation analysis of CCR7, L-selectin, CD44v6, MMP9expressionThere were positive correlation between the expression of CCR7and L-selectin, CCR7and MMP9, L-selectin and CD44v6, CD44v6and MMP9, L-selectin and MMP9in colorectal carcinoma tissues (P<0.05).Get the conclusion by this experiment:1The high expression of CCR7, L-selectin, CD44v6, MMP9in colorectal carcinoma tissues could involve in intestinal mucosa carcinogenesis.2The expression of CCR7, L-selectin, CD44v6, MMP9in metastatic tumor tissue were higher than in colorectal carcinoma tissues, and the expression of CCR7, L-selectin, CD44v6, MMP9in cases with lymph node metastasis were higher than that without lymph node metastasis, which indicate that the expression of CCR7, L-selectin, CD44v6, MMP9could involve in node metastasis of colorectal carcinoma.3There were positive correlation between the expression of CCR7and L-selectin, L-selectin and CD44v6in colorectal carcinoma tissues, they are the key factors of the lymphoid homing, whether lymph node metastasis of colorectal carcinoma is simulate the process of lymphoid homing, we need to prove by further study. The positive correlation between the expression of MMP9and CCR7, CD44v6in colorectal carcinoma tissues, which indicate that MMP9collaborative above factors in the lymph node metastasis of colorectal carcinoma.