Cyclohosphamide-induced Rat Ovarian Damage And Expression Of Androgen Receptor In The Damaged Ovaries

Objective:To investigate the expression of androgen receptor(AR) in the damaged ovaries for the exploring the role of AR in the process of cyclohosphamide-induced rat ovarian damage.Methods:30adult SD rats with normal estrous cycle were randomly divided into two groups:the blank matched control group, cyclohosphamide (CTX) group. Two groups of rats were subjected to intraperitoneal injection of a single-dose cyclophosphamide (75ml/kg) and saline, respectively. Increasing degree of rat weight, change of ovary and uterus wet weight, numbers of the follicle and histomorphological features of ovary were observed. ELISA (enzyme linked immunosorbant assay) was used to analyze serum level of FSH (follicle-stimulating hormone) and E2(estradiol) among the two groups.The expression of AR protein was evaluated by immunohistochemistry,and ARmRNA expression determined by Real-Time PCR in rat ovaries.Results:1. The average ovarian weight and the increasing degress of the rat weight of CTX group were lower than that of the control group(P<0.05).The difference of uterus wet weight between the two groups was not obvious(P>0.05).2. As compared with the control group, FSH level of CTX group significantly increase, and E2level of CTX group significantly decreased(P<0.05).3. As compared with the control group, CTX treatments produced a significant reduction in the number of primordial follicle, preantral follicle,antral follicle and the total follicle (P<0.05).But the reduction of second follicle number was not significant (P>0.05).4. The express of AR protein in the ovaries of CTX group was significantly lower than that in the control group(P<0.05).5. The express of ARmRNA in the ovaries of CTX group was significantly lower than that in the control group(P<0.05).Conclusion:1.Intraperitoneal injection of a75ml/kg single-dose cyclophospha-mide can cause obvious ovarian damage.2. CTX can cause a lower expression of the AR, which suggests that AR may play a role in the process of cyclohosphamide-induced rat ovarian damage.