Expression And Clinical Significance Of Intercellular Adhesion Molecules-1and E-selectin In Esophageal Squamous Cell Carcinoma

Objective and BackgroundEsophageal cancer is the most common malignant tumor in China, its mortalityrate ranks fourth in malignant tumor. Early patients often lack of obvious symptoms,most advanced patients died of local infiltration, distant metastasis and postoperativerecurrence. Tumor invasion and metastasis are the main cause of death in patientswith Esophageal cancer. Cell adhesion molecule play a decisive role in the process oftumor invasion. Intercellular adhesion molecule-1(ICAM-1) is a very important celladhesion molecule, it can adjust the combination of cells and cells, cells andextracellular matrix, it is thought to play an important role in tumor invasion andmetastasis. E-selectin is an important member of the selectin family, it can mediateadhesion of platelets, endothelial cells and neutrophils, monocytes, lymphocytes andtumor cells, and it is closely related to tumor invasion and metastasis.Research showsthat high ICAM-1or E-selectin expression in gastric cancer, liver cancer, lung cancerand other tumor tissue is significantly associated with invasion and metastasis and isexpected as a predictor of metastasis, recurrence and prognosis. The expression andclinical significance in esophageal carcinoma were little studied at home and abroad.The expression of E-selectin mRNA and ICAM-1mRNA in esophageal squamous cell carcinom（aESCC）and adjacent normal mucosa tissues was detected byreal-time PCR method. E-selectin and ICAM-1protein levels were determined byimmunohistochemical pv-9000method.To explore their relationship with invasionand metastasis, we combined with clinical pathological factors. It provide atheoretical basis for clinical targeted anticancer therapy and effective prognosisjudgement.MethodsA total of58esophageal carcinoma tissues were collected in Shandong tumorHospital from2012January to May. Esophageal carcinoma tissues was squamous cellcarcinoma by pathological diagnosis, all patients did not accept any anticancertreatment before operation.At the same tim, the corresponding adjacent normal tissues(distance carcinoma≥5cm) as control. The expression of E-selectin mRNA andICAM-1mRNA was detected by real-time PCR method. E-selectin and ICAM-1protein levels were determined by immunohistochemical pv-9000method.Therelation between E-selectin、ICAM-1expression and the clinicalpathological featureswas analyed. The relation between E-selectin, ICAM-1expression was also analyed.Results1The expression of ICAM-1mRNA: The expression of ICAM-1mRNA weresignificantly higher in esophageal squamous cell carcinoma than that in normalmucosa(P＜0.01). The expression of ICAM-1mRNA significantly increased inpatients with deeper invasion，clinical stage Ⅲ, lymph node metastasis (P<0.05).There was no statistically significant difference in gender, age, tumor location,pathological types, tumor size,degree of differentiation (P>0.05).2The expression of ICAM-1protein: The positive rate of ICAM-1protein inesophageal squamous cell carcinoma was53.4%(31/58)，significantly higher thanthat in normal mucosa0.0%,(P＜0.01).The deeper is the invasion depth of ESCC,the higher is expression of ICAM-1protein. The expression of ICAM-1protein in lymph node metastasis and clinical stage Ⅲ were significantly higher than that in nolymph node metastasis and clinical stageⅠ～Ⅱ (P<0.05). No positive correlationwas found between the expression of ICAM-1protein and gender, age, tumor location,pathological types, tumor size, degree of differentiation (P>0.05).3The expression of E-selectin mRNA: The expression of E-selectin mRNA weresignificantly higher in esophageal squamous cell carcinoma than that in normalmucosa(P＜0.01). The expression of ICAM-1mRNA significantly increased inpatients with deeper invasion，later clinical stage, lymph node metastasis (P<0.05).There was no statistically significant difference in gender, age, tumor size, tumorlocation, pathological types, tumor size, degree of differentiation (P>0.05).4The expression of E-selectin protein: The positive rate of E-selectin protein inesophageal squamous cell carcinoma was67.2%（39/58）,significantly higher than thatin normal mucosa (0.0%, P＜0.01). The deeper is the invasion depth of ESCC, thehigher is expression of E-selectin protein. The expression of E-selectin protein inlymph node metastasis and clinical stage Ⅲwere significantly higher than that in nolymph node metastasis and clinical stageⅠ～Ⅱ (P<0.05). NO positive correlationwas found between the expression of E-selectin protein and gender, age, tumorlocation, pathological types, tumor size, degree of differentiation,(P>0.05).5The relationship between E-selectin and ICAM-1:There was a significantpositive correlation between E-selectin mRNA and mRNA (r=0.760，P＜0.01).E-selectin protein also had a positive correlation with ICAM-1protein in esophagealsquamous cell carcinoma (r=0.306，P<0.05).Conclusions1The expression of E-selectin and ICAM-1significantly increased in esophagealsquamous cell carcinoma, they may be related to occurrence of esophageal squamouscell carcinoma.2The high expression of E-selectin and ICAM-1in in ESCC tissues weresignificantly increased and related to the invasion and lymph node metastasis.The enhanced E-selectin and ICAM-1may serve as auxiliary indexs judging diseaseprogression, invasion, metastasis and a new biological target for the treatment ofesophageal cell carcinoma.3There was a significant positive correlation between E-selectin and ICAM-1.It suggesting that both may jointly promote and accelerate tumor progression in thedevelopment process of esophageal squamous cell carcinoma, but the specificmechanisms still need further research.