Study Mechanism Of Water Metabolism About Dampness Obstructing Spleen-stomach Syndrome On Qualitative And Quantitative Expression Of Aquaporins (AQPs)

Purpose:Comb theoretical knowledge of the syndrome of Dampness Blocking Spleen and Stomach, study the qualitative quantitative expression characteristics of subtypes (AQPs) water channel protein, part reveal the pathology mechanism of moisture trapped spleen and stomach syndrome and flat stomach powder treatment effect mechanism.Methods:1.Through literature research, combing the characteristics of wet trapped spleen and stomach syndrome and the treatment regularity of drug2.joint the inside and outside wet factor together (lived wetlands for a long time, excessive wet outside;improper diet, affect-mind dissatisfaction, excessive wet inside), established the subjects of wet spleen and stomach syndrome model by SD rat.3.using immunohistochemical (IHC) detection of AQPs in the digestive tract anatomy of the related parts of the distribution and expression, enzyme league immune method to determine the content of gastric tissue AQPs (ELISA).Result:1.Wet trapped taste card to be born inside wet chaotic, poor angry machine, coke taste trapped as the main cause; To taste the dirty as the main disease; Has given priority to with wet, in the disease of sex is easy and its evil, can characteristics of thermalization cold; In an attempt to physical contrast with the forces of evil are related, more touching refractory characteristics; On syndrome, wet as its height pixu (spleen deficient) as the main performance, has a certain space and time characteristic; On the treatment for wet pixu (spleen deficient) the main pathogenesis, ingredients of spleen qushi more genera.2.AQP0,1,2,3main expressed in gastric tissue mucosa layer, outer membrane expression may have suspected, little expression in submucosa and muscular layer.3.AQP0,1,2,3have differences in distribution in the gastrointestinal tract. Under normal circumstances (blank), AQPO mainly expressed in the middle of the gastric cardia and gastric body, compared with blank group,model group has a tendency to cut; AQP1 mainly expressed in middle gastric body and colon, compared with blank group, model group has a tendency to rise in gastric cardia jejunum, rectal,has a tendency to cut in the stomach; AQP2is mainly in the middle of the gastric cardia, gastric body and rectum expression, the expression of AQP2in gastric cardia, compared with model group and blank group has a tendency to increase, in the stomach, jejunum and colon, rectum, has a tendency to cut; AQP3expression mainly in the middle of the gastric cardia, gastric body, and AQP3in the expression of gastric cardia in the stomach, jejunum, compared with model group and blank group has a tendency to increase.4.The content of AQPO in colon, compared with blank group (x±s), model group,Pingwei Dosage group, blank values to medicine group, natural recovery group were lower and there were significant differences between groups (P<0.01).5.in terms of content of AQP1in the stomach, compared with the blank group (x±s), model group slightly drop, flat stomach rise in value, but no statistical significance (group (P>0.05), the blank value increase, there is difference between groups (P<0.05), numerical lower natural recovery group, there were significant differences between groups (P<0.01).6.in terms of content of AQP2in colon, compared with blank group (x±s), model group, flat stomach, blank values to medicine group, natural recovery group were lower and there were significant differences between groups (P<0.01).7.in terms of content of AQP3in jejunum, compared with blank group(x±s), model group, the flat blank, group and drug group were increased and there were significant differences between groups (P<0.01), natural recovery group number increases, there is difference between groups (P<0.05)Conclusion:1.The Pathogenesis characteristics of wet trapped spleen and stomach syndrome is similar between the Animal model and the patient, the animal model preparation method is worthy of learning.2.Confirmed AQP0,1,2,3are mainly expressed in gastrointestinal mucosa, gastrointestinal mucosa of water absorption and metabolism play an important role.3.AQP0, AQP1, AQP2may be one Of the molecular mechanismthe which made the Spleen and stomach stranded. the qi and water stoped on syndrome of Dampness Blocking Spleen and Stomach.4. AQP1, AQP2is likely to be one of the molecular mechanism about scattered wet promoting qi and Eliminate stagnant.