Basic Material Research About Astragalus Euonymus Alatus Mixture Improve Diabetes In Mice

ObjectiveBy using real-time quantitative per techniques to find how astragaluseuonymus alatus mixture Play the contral role to change kkmice blood glucose level. Explore the function of the material basis. At the same time Combined1H-NMR experiments, From the perspective of metabolomics looking for the effector molecules of astragaluseuonymus alatus mixture improve Diabetes in mice, and through the different pair wise comparison plotted1H-NMR spectra, And then analysis the metabolites Changes in overall trend.Methods1, Realtime fluores-cence quantitative PCR experiment to detect predicted target genes level in mice kidney tissue:First, create a type2diabetic mice models, observe the astragaluseuonymus alatus mixture how to change the blood glucose level in mice.When the official experiment start, give every group the corresponding medicine, After six cycles use the RT-PCR technique to detect the kidney tissue. Preliminary, the research group predicted potential pharmacological targets through the network, the six predict potential target gene is HMGCR、RBP4、REN、ESR1、SHBG、IL-2.2、Urine metabolomics testing result:For6weeks to collect animals urine, through H-NMRsPectrum test, Obtain spectra of urine metabolic component, then compare and analyze the map, According to the peak to find molecules that may play an important role, and then analysis the metabolites Changes in overall trend.Results1、The astragaluseuonymus alatus mixture can change the mice blood glucose levels at the four week. The glucose values from33.3mmol/L down to21.9mmol/L, and remained at this level in the fifth to sixth weeks.2、The result of RT-PCR experiment:Comparison astragaluseuonymus alatus Mixture group with model group, There have significant differences in the expression of gene ESR1and Ren. Comparison positive group with model group there have significant differences in the expression of gen HMGCR and REN.3, Metabolomics results:Comparison the data of each two group, find differences between the two groups after comparison the peak, And after compared to the chemical shifts, find each peak represents which chemical substances. Comparison astragaluseuonymus alatus mixture group with model group, differences in gredients are:malic, a-keto glutaricacid, histidine, betaine; Comparison astragaluseuonymus alatus mixture group with positive group, differences in gredients are:tryp tophan, ascorbic acid, methionine, aspartic acid, allantoin; comparison model group with Positive group, differences ingredients are:Proline, citrulline, threonine, isoleucine, histidine.4、The PC images and PC-Loading diagram showed that each information point can be observed within the90%confidence intervalarea, indicating a successful model was established.Each point fluctuations also showed there is a difference in the metabolites, Drug effects are different for each group.5、PLS-DA data Analysis:The information of various sites further illustrate the differences of metabolic component in each group.ConclusionThrough animal experiments, confirm astragaluseuonymus alatus mixture can change the blood glucose level to a certain extent, And there is a better trend to lower blood glucose levels.