Study Of Radix Platycodon Grandiflorum Extract Polysaccharides And Its Quality Control

Platycodon grandiflorum(Jacq.)A.DC.is the dry root of Radix Platycodonis.[1].. Bitter, pungent, flat, lung meridian tropism. Clinical for lung undeclared cough sputum, chest tightness, sore throat hoarse disease. In this paper, Platycodon grandiflorum and fluidextract for quality control purposes, the saponins in Platycodon grandiflorum and multi carbohydrate ingredients are as follows:Study on Extraction and separation of Platycodon root polysaccharide: The water extraction and alcohol precipitation method is a water soluble neutral Campanulaceae, UV spectrum analysis showed that the polysaccharide did not contain protein, nucleic acid and polypeptide; infrared spectra shows that the polysaccharide has the absorption peak, and confirmed that Radix Platycodonis with alpha and beta two configurations of glycoside key, and to the pyranose. Determination of polysaccharide molecular weight, with different molecular weight dextran were measured, the water-soluble polysaccharide molecular weight was3993Da; Gas chromatography demonstrated that the polysaccharide containing glucose,rhamnose, xylose. The molar ratio is3.46:11.40:3.16.Content determination of Radix Platycodon grandiflorum, pieces:To simplify the experiment on the2010version of "Chinese Pharmacopoeia" in "for the content determination of Radix Platycodon grandiflorum under test solution preparation method", delete the latter part of the complex process of column. The experimental period is shortened while avoiding the use of chloroform, perfect for the quality standard of Radix Platycodon grandiflorum provided plenty of scientific data.Study on the quality standard of Platycodon grandiflorum. extract stream:The experimental set up of Platycodon grandiflorum saponins content flow in the extract of TLC method and Platycodon saponins, polysaccharides determination method, to study the extractum Platycodi liquidum fingerprint by high performance liquid chromatography method, and the method validation, fingerprint analysis method of the results Biao Mingjian set the precision, reproducibility and stability, fingerprint study complies with the technical requirements.Through the above research, to further improve the quality standard of Radix Platycodon grandiflorum, tablet and liquid extract, better control of its internal quality. Hope that in future studies, to further separation of saponins in Platycodon grandiflorum, polysaccharides and other ingredients, and other components outside of platycodin D are identified.