The Study On The Pharmacy And Pharmacodynamics Of Wenzhong Yiqi Capsule

Objective:1, On relieving Wenzhong Yiqi capsule and its preparation technology and quality standard were studied, and the preliminary investigation on the stability of cough and asthma.2, Observe the anti-ulcer function of the Wenzhong Yiqi capsule and preliminary study the mechanism by establishing pyluros ligation-induced gastric ulcer model; Using the motheds of mouse auricle swelling induced by xylene, hot plate stimulation and acetic acid writhing to proceed anti-inflammatory and analgesic experiment. Observe the effect of the anti-inflammatory and analgesic.Methods:1, Preparation process stability and pilot study in Wenzhong Yiqi capsule. Investigate its moisture, heavy metals, arsenic salt content, disintegration time, load difference and hygiene, Using the thin layer chromatography (TLC) to the prescription of atractylodes, determination of composition of coptis chinensis and microscopic identification method is applied in the work of party atractylodes, poria cocos, observing at the same time using thin layer chromatography (TLC) to identify the ingredients of the atractylodes, rhizoma coptidis for Wenzhong Yiqi capsules.The stability study of accelerated test method at room temperature and sample observation method.2, Use pyluros ligation method to duplicate rat gastric model to observe the inhibition of the Wenzhong Yiqi capsule on ulcer area and ulcer index. Detect the content of gastric juice quantity, gastric acidity, NO, proteinase activity of gastric juice. The content of GAS, PGE2, and the content change of the IL-1and EGF in serum, the content of MDA, the activity of SOD and H+K+-atpase activity, the content change of SS. Using the motheds of mouse auricle swelling induced by xylene, hot plate stimulation and acetic acid writhing to proceed anti-inflammatory and analgesic experiment, determination of mice ear swelling degree, the size of the pain threshold and. The number of twist body.3, With the volume of the maximum concentration of mice by gastric, Observation for7days of diet and activity in mice, and presence of death.Results:1, Wenzhong Yiqi capsule preparing process for: atractylodes, tuckahoe have strong powder, crushed into fine powder medicine, to ensure effective component loss is small, and the effect of preparation materials; Ginseng, pinellia, dried ginger, broil licorice, rhizoma coptidis, radix scutellariae, jujube seven herbs according to the clinical application of decoction method of preparation. Water decoction extraction twice, filtering, extracted twice into the same beaker, condensed into a cream, dried, crushed into fine powder; Atractylodes, tuckahoe powder and paste powder, sieving and mixed granulating. Capsule, packaging, Co60irradiation sterilization.2, Quality standards: the capsule effective composition content meet the national standards.contents for the browns powder; Micro, bitter taste. Of mold and yeast content is not more than100per gram, did not check out, living mites, staphylococcus aureus. e. coli, verdigris pseudomonas bacteria.3, Stability testing results, the appearance of reagent, identification, inspection and hygiene inspection of various indexes such as six months were not obvious changes.4, pharmacodynamic experiment shows, The gastric ulcer experiment, Compared with blank group, model group H+-K+-atpase activity and MDA content increased significantly(P <0.01), SS、PGE2and SOD content decreased significantly (P <0.05or P <0.01), Compared with model group, Wenzhong Yiqi capsules can significantly reduce the rat gastric ulcer area and ulcer index, reduce the H+-K+-atpase activity and MDA content and increase of the SS and SOD content in stomach tissue (P <0.05or P <0.01),Wenzhong Yiqi capsule can obviously decrease the rat gastric juice, NO content(P<0.01), reduce the gastric acidity and pepsin activity(P <0.01), increased gastric secrete element, the content of PGE2in serum(P <0.05); In anti-inflammatory and analgesic experiment, compared with the blank group Wenzhong Yiqi capsule can reduce the degree of mouse auricle swelling induced by xylene, increase the pain threshold in mice (P <0.01, P <0.05).5, Wenzhong Yiqi capsule of the mouse stomach lavaging of maximum dose of43.91g/kg, equivalent to512.31times the amount of clinical adult, in the observation period, it had a normal activities, the water eating normal color luster, no abnormal phenomenon. So that, the medicine clinical oral medication safety.Conclusion:1, Wenzhong Yiqi capsule pilot study showed the drug preparation, good stability, quality inspection completely compliance with national standards. Wenzhong Yiqi capsule stability test results consistent with preclinical drug quality standard requirements, the preparation in the quality of basic stability during storage, storage period is tentatively scheduled for24months.2, Wenzhong Yiqi capsule can effectively to pyloric ligation model of gastric ulcer of rats whith good treatment and prevention of the role, have anti-inflammatory, and analgesic action.3. Acute toxicity test, the dosage is far higher than that of clinical dosage, often mice did not appear abnormal behavior, yiqi capsule in temperature that clinical medication safety.