| Objective:This study was to investigate the intervention of Jia-Ji EA, compared with single ganglioside saliva acid, on oligodendrocytes differentiation and its probable mechanism on remyelination and motor functional recovery of acute spinal cord injury in rats by detecting the expression of olig2, sox10and myelin basic protein (MBP), observing morphologic changes of myelin sheath and BBB scores.Methods:144male SD rats were randomized into the sham control group(Sham group), the model control group(model group), single ganglioside saliva acid group (GM1group) and Jia-Ji EA group (EA group), and each group was further divided into day1, day7, day14post-operation(n=12). In our study, rats in the sham group were operated by vertebral plate resection only, in other three groups, T9-T10spinal segment were acute moderate damaged by NYU impactor. GM1was injected intraperitoneally with the dose of10mg/kg once a day. EA (disperse-dense wave,2/100Hz,1mA) was applied to acupoints around the damaged segment once a day for20min. After the treatment in each time point, the following steps were given:①BBB locomotor rating scale was handled prior to be executed in order to observe motor functional recovery of rats hind limbs;②Luxol Fast Blue (LFB) myelin staining was used to observe the myelin sheath regeneration with using Imagepro Plus software;③estern Blot was used to detect the expression of olig2, sox10, MBP to observe the intervention of Jia-Ji EA on oligodendrocytes differentiation and remyelination.Results:①ay1post-SCI, there were no statistical differences of BBB scores between the two intervention groups and the model group. Day7and14post-SCI, BBB scores increased gradually in the two intervention groups, and there was a significant difference compared with the model group (P<0.01), and between the two intervention groups (P<0.01);②FB myelin staining showed that color of large-area in white matter of damaged spinal cord was lighter, interval became sparse with amount of cavities, and arrangement became irregular. Day1post-SCI, IOD values in the model group were significantly decreased compared with the sham group (P<0.01), but no significant difference compared with the two intervention groups and between the two intervention groups. Day7and14post-SCI, the number of myelin sheath gradually increased in two intervention groups, with a significant difference compared with the model group(P<0.05or P<0.01) and between the two intervention groups(P<0.01);③㊣n model group, the expression of olig2and sox10increased significantly compared with the sham group in each time point(P<0.01). In the two intervention groups, the two proteins expression got a peak at day7post-SCI and descended slightly at day14post-SCI, with a significant difference compared with the model group (P<0.01or P<0.05) and between the two intervention groups (P<0.01or P<0.05); In model group, MBP expression increased gradually and the differences in each time point were all significant compared with the sham group (P<0.14or P<0.05). In the two intervention groups, the expression got a peak at day14post-SCI and there was a significant difference compared with the model group (P<0.01) and between the two intervention groups (P<0.01) except day1post-SCI.Conclusions:①Jia-Ji EA can promote the motor functional recovery of spinal cord injury in rats;②Jia-Ji EA can promote the myelin regeneration of spinal cord injury in rats;③The mechanism of Jia-Ji EA on remyelination and motor functional recovery of spinal cord injury in rats probably is its intervention on oligodendrocytes differentiation by increasing the expression of olig2and sox10, the oligodendrocytes transcription factors;④Jia-Ji EA may have the same effective targets with GM1. |
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