Sub Typing Of TNBC And Research Of Its Clinical And Pathological Characteristics

Background and purpose：Triple-negative breast cancer（TNBC）is refers to the absence of hormonereceptor （include ER, PR） and lack Her-2protein over expression or geneamplification. It can be divided into basal-like TNBC（BL-TNBC）and non-BL-TNBCby uniting the detection of EGFR and CK5/6through IHC. Tow subtype have quitedifferent clinical，pathological and prognosis characteristics. To explore the clinical，pathological characteristics between them, we analysis81cases of TNBC to analyzethe clinical pathological factors.Method：We studied a cohort of81female patients with TNBC，diagnosed betweenJanuary2010and January2013at the China-Japan Union hospital of Jilin University.All patients were confirmed to be TNBC by IHC with complete clinical andpathological data. Cases were divided into BL-TNBC subgroup with EGFR andCK5/6one or both positive， both negative cases divided into non-BL-TNBCsubgroup. We used the SPSS17.0statistical software to analysis the age at diagnose，menstrual status，tumor size，lymph node metastasis，clinical stage，pathological type，histological grade，and the expression of Ki-67and P53between them. We alsoconducted a subgroup analysis on the BL-TNBC group，to explore the effects of basalmarkers（EGFR， CK5/6）on clinical pathological factors. We applied t test onmeasurement data，chi-square test on enumeration data to compare significantdifference between groups，with P <0.05considered to be statistically significant.Results：Of the81TNBC cases involved in this study，BL-TNBC represent60.5％ofTNBCs. Age ranging from30to75，median50，most of its pathological type is invasive ductal carcinoma（86.4％）. There is no statistical difference exists in the ageat diagnose，menstrual status，pathological type，lymph node metastasis，P53expressing level between the two subtype（P＞0.05）. BL-TNBC patients are morelikely to be larger in tumour size（diameter＞20mm，61.3％vs31.3％，P＜0.05）.Allpatients are of histological grade Ⅱand Ⅲ，BL-TNBC has higher histological grade（grade Ⅲ，71.4％vs46.9％，P＜0.05）.BL-TNBC patients are also have higherexpressing level of Ki-67（51.01％±3.19％vs36.25％±4.44％，P＜0.05）.Furthermore，BL-TNBC always have later clinical stage（P＜0.05）.We also carried a subgroup analysis on the49cases of BL-TNBC subgroup. Thepositive rate of EGFR is48.9％. EGFR positive group likely to be younger at age ofdiagnosi（smedian，45vs49.5）. There is no statistical difference exists in the menstrualstatus，tumor size，pathological type，histological grade，expression of Ki-67betweenthe two group（P＞0.05）.EGFR positive group is more likely to has lymph nodemetastatic（65.2％vs50.0％），higher number of positive lymph node（＞10,34.8％vs3.8％，P＜0.05）.Also，EGFR positive group has later clinical stage（P＜0.05）.While the positive rate of CK5/6is83.7％. We didn’t conclude any statisticaldifference on the age at diagnose，menstrual status，tumor size，lymph nodemetastasis，clinical stage，pathological type，histological grade，and the expressionof Ki-67and P53between the CK5/6positive and negative group.Conclusion：1、Basal-like TNBC account for60.5％of TNBCs.2、Basal-like TNBCs arelarger in tumour size，have higher histologic level and Ki-67expressing level，andlater clinical stage.3、In basal-like TNBC，EGFR positive is associated with youngerage at diagnose，higher lymph node metastatic rate and later clinical stage.