| This study aims to explore the inhibitory effect on liver cancer cells BEL-7402of PanaxNotoginseng Saponin（sPNS）in vitro and briefly discuss the effect and the mechanism of itseffect in mouse in situ transplantation live cell carcinoma.Methods1.Inhibitory effect of PNS on human hepatocellular carcinoma cell BEL-7402in vitro.Cell proliferation was detected by MTT method, following drawing the humanhepatocellular carcinoma BEL-7402cell growth curve and determine the cell concentrationfor further investigation. Different concentrations of PNS (5×10-5、2.5×10-5、1×10-5、7.5×10-6、5×10-6、2.5×10-6、1×10-6、5×10-7、1×10-7and0mol/L) were added in BEL-7402cells, and cellproliferation was detected at different time point (24,48,72,96and120h).2. Inhibitory effect of PNS on mouse in situ transplantation liver cell carcinoma. Mouseorthotopic hepatoma model was established by using in situ injection method on day0. Thenthe mice were randomly divided into5groups,15in each group, respectively, includingnormal animal group, animal model group, high dose of PNS group (100mg/kg), low dose ofliquid medicine group (50mg/kg) and positive drug group (20mg/kg). At the second day themice were administered the drugs orally,1times a day, for15days.24h after the lastadministration, tumor inhibition rate was calculated, the relative phase distribution andproliferation index of cancer cells was determined by flow cytometry and morphologicalobservation of liver and tumor tissue were detected.ResultInhibitory effect of PNS on human hepatocellular carcinoma cell line BEL-7402in vitro:PNS could inhibit the proliferation of BEL-7402cells.1.48h after treatment with PNS, the cell growth inhibition rates were8.57%,13.61%,28.25%,29.02%,33.06%,36.90%,42.55%,49.44%and52.09%mol/L, Amongthem,5×10-5the inhibition rate reached50%;72h after administration, the inhibitory rateswere17.63%,25.01%,45.39%,51.26%,57.22%,58.81%,59.56%,59.92%and70.07%，fromlow dose of PNS to high dose.Among them, at the concentration of2.5×10-6,5×10-6,7.5× 10-6,1×10-5,2.5×10-5and5×10-5mol/L,the inhibition rate reached50%;96h afteradministration, the inhibitory rates were16.64%,26.57%,32.93%,41.57%,43.41%,42.76%,49.72%,55.56%and68.94%，from lowdose of PNS to high dose. The result showed that the different concentrations of PNS havedifferent inhibitory effect on the growth of BEL-7402cells, with the increase of drugconcentration, the inhibition effects on tumor cells increased gradually, and the optimalreaction time was72h.2. Inhibitory effect of PNS on mouse in situ transplantation liver cell carcinoma. Thetumor inhibition rate of the high dose (100mg/kg) of PNS group is45.06%, and the tumorinhibition rate of the low dose (50mg/kg) of PNS group was31.70%. Compared with the lowdose of PNS group, the liver cancer cells in PNS high dose group were significantly arrestedin S phase and cell proliferation was inhibited. The result indicated that high dose of PNScould inhibit in situ transplantation liver cell carcinoma in vivo.Conclusion1. PNS could inhibit the growth of human hepatoma BEL-7402cells in vitro. Thedifferent concentrations of PNS have different inhibitory effect on the growth of BEL-7402cells, with the increase of drug concentration, the inhibition effects on tumor cells increasedgradually; and the optimal reaction time was72h.2. PNS could inhibit mouse in situ transplantation liver cell carcinoma. The tumorinhibition rate of the high dose (100mg/kg) of PNS group is45.06%, and the tumorinhibition rate of the low dose (50mg/kg) of PNS group was31.70%. Compared with the lowdose of PNS group, the liver cancer cells in PNS high dose group were significantly arrestedin S phase and cell proliferation was inhibited. |
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