Expression And Function Anaysis Of FSCN1Following Spinal Cord Injury

Background and objective：Spinal cord injury is always the emphasis of medical research. Withthe mushroom growth of industry, transportation industry and the upgradeof incident of violence in local area, the morbidity of spinal cordtransection rise yearly, but mechanism of apoptosis and nerveregeneration repair has not yet been fully elucidated. The study will makethe spinal cord cross-sectional damage model of SD rats, then verify theexpression changes of FSCN1after spinal cord transection injury throughimmunohistochemical method and western blotting,discuss FSCN1’seffect after spinal cord injury in order to provide new ideas to the researchof treatment and neural functional recovery after spinal cord injury inclinical.Method：The experimental subject of this study are SD adult rats.Aftersuccessfully made the spinal cord cross-sectional damage model,36adultSD rats were randomly divided into normal control group and group ofspinal cord injury,and then the damage group will be divided into12h,24h,72h,7d and14d groups according to the time of drawmaterials.Under the microscope,the T10segment were traversed completely with knife in spinal cord injury group.Evaluate the hind limbsmotor function of experimental subject in each group with BBB ratingscale.Take the relevant spinal segments,he distance of which is5mm tothe center of the damage area in each group,as the research samples,thentest the expression changes of FSCN1after spinal cord transection injuryin different time points through immunohistochemical method andwestern blotting,finally analysis the correlation compared with the controlgroup.Result：1.The study successfully make the spinal cord cross-sectionaldamage model of SD rats,the rat’s hind legs become paraplegia afterinjury.The behavioral score of the12h,24h injury group are all0,somerat’s hind limbs has motor function in the72h,7d and14d injurygroups(the score of BBB has raised to2.6points).2.The western blotting shows the obviously up-regulated expressionof FSCN1in24h after the spinal cord transection injury,and then gradualdecline after24h which is still higher than normal control group.Theimmunohistochemical results prove that FSCN1express in spinal cordgrey matter in normal tissue,the cytoplasm of gray matter anterior hornneurons and white matter of spinal cord glial cells in SCI tissue,andexpress quantity reaches its peak in24h. Conclusion：The feeling and motor function of hind limbs have no significantrecovery in14d after spinal cord transection injury completely.Accordingto the expression changes of FSCN1after injury,we can analysis thatFSCN1may play an important role in the process of apoptosis and nerveregeneration or repair.