Electroacupuncture On Apoptosis-related Factors And Nerve Regeneration After Spinal Cord Injury

literature review and experimental research.First Section:literature ReviewThe paper have three literature summarys. The first article reviews the research progress of the spinal cord injury (SCI) from western treatment and treatment of traditional Chinese medicine (TCM) in the resent years at home and abroad. The second article introduces the relationships among the apoptosis myelin sheath changes,mitochondria and spinal cord injury; The third article summarizes the clinical research and experimental surveys on the spinal cord injury (SCI) in rats with the acupuncture and moxibustion.Second Section:Experimental ResearchObjective:To explore mechanism of the therapeutic and protective functions with electroacupuncture (EA) to the spinal cord injury (SCI) in rats.Methods:We divided the rats into four groups randomly:the Model Group, the Blank Group, the Drug Group, the EA group. We use the modified Allen's methods to make into a spinal cord contusion of the model rats. (1) comprehensive evaluation on movement,sensation,reflexion,the neural functions of SCI by using the CBS. (2) To detect the TNFR1,Fas of the local i njuried tissues by photo analysis which were fixed, embed, sliced, processed, dyed. (3) The local injured tissues of the Model Group, the Blank Group, the EA Group and the Drug Group were removed to detect the contents of Fas,TNFR1,Calpastatin,Caspase-3 mRNA through RT-PCR. (4) To observe the ultrastructural changes of axons and mitochondria in spinal cord tissues after SCI with transmission electron microscopy(TEM)Results:after 6h,1d,7d of SCI, (1) Compared with the Model group, the scores of CBS in the EA Group and the Drug group were significant differently (P<0.01); Compared with the Blank Group, the scores of CBS in the Model Group was significant differently (P<0.01); (2) The contents of Fas, TNFR1 in the local injuried tissues were decreased significantly in the EA Group and the Drug Group on the 6h,Id and the 7d after SCI. Compared with the Model Group, there were statistical significance in the EA Group and the Drug Group (P<0.01). Compared with the Drug Group, the Fas,TNFR1 contents of local injuried tissues were increased slightly in the EA Group and there was no statistical significance between the Drug Group and the EA Group (P>0.05). (3) The Calpastatin mRNA content of local injuried tissues were increased significantly in the EA Group and the Drug Group on the 6h,1 d and 7d after SCI. Compared with the Model Group, statistical significance were found in the EA Group and the Drug Group (P<0.01). There was no statistical significance between the Drug Group and the EA Group (P>0.05). (4) The contents of Fas,TNFR1,Caspase-3 mRNA in local injuried tissues were decreased significantly in the EA Group and the Drug Group on the 6h,1 d and 7 d after spinal cord injury. Compared with the Model Group, statistical significance were found in the EA Group and the Drug Group (P<0.01). There was no statistical significance between the Drug Group and the EA Group (P>0.05). (5) Compared with the Model Group, the myelin sheath were more compact, degeneration were reduced, mitochon -dria were seen in axons, but still exited separation of myelin board layer, edema, solidification and degeneration of part axons.Conclusion:EA has the therapeutic and protective effects on the SCI. It maybe works through reducing the contents of Fas,TNFR1 in different times to inhibit apoptosis,reduce the formation of the scares due to the fibroblasts substantial accumulation to protect the nerve cells after SCI;EA has the protective effects may also be increasing the contents of Calpastatin and decreasing the contents of Caspase-3 to inhibit apoptosis, reduce the inflammatory, restrain thr Calpain activity, reduce myelin depigmentation, save mitochondrial function on the SCI in rats.