Association Of Helicobacter Pylori Infection And Polymorphisms Of PSCA, PLCE1and PRKAA1with Gastric Cancer Susceptibility

Objective:By testing the polymorphism of PSCA(rs2294008), PLCE1(rs3203713, rs11599672) and PRKAA1(rs13361707) and Helicobacter pylori infection among gastric cancer, precancerous and normal control group in Gansu Han population, to investigate the association of them with gastric cancer susceptibility.Methods:A total of747cases were divided into three groups, including217gastric cancers,102precancerous and428normal controls, all cases were diagnosed by pathology. Clinical datas were collected including gender, age, family history of gastric cancer, epidemiological data etc.. The tests of SNP types were used by the method of multiplex single base extension (Multiplex SNaPshot). Detection of H. pylori infection was used by urea [C13] breath test. The data was analysed through X2test, single factor variance analysis and logistic regression.Results:We found that the risk of gastric cancer for rs2294008CT/TT genotype carriers was1.778times higher than that of CC genotype carriers (OR,1.778;95%CI,1.272-2.484;P=0.001); rs11599672AC/CC genotype carriers1.981times higher than AA genotype carriers (OR,1.981;95%CI,1.423-2.758;P=0.000) and the malignant potential of precancerous lesions1.960times higher than AA genotype carriers (OR,1.960;95%CI,1.216-3.159; P=0.005);rs13361707CT/CC genotype carriers1.587times higher than TT genotype (OR,1.587;95%CI,1.085～2.322; P=0.017). Logistic regression analysis showed that rs2294008CT/TT genotype, rs11599672AC/CC genotype, rs13361707CT/CC genotype, drinking, family history of gastric cancer and H. pylori infection were associaed with the risk of gastric cancer, and there were additive interaction for the risk of gastric cancer between these three kinds of genotypes and alcohol drinking, family history of gastric cancer and H. pylori infection. Both the genotype of rs11599672AC/CC and positive family history were associated with precancerous lesions of the malignant potential, and the two together could make the precancerous lesions of the malignant potential increase. The risk of gastric cancer was6.792times higher compared individuals who carried rs2294008CT/TT, rs11599672AC/CC and rs13361707CT/CC genotype simultaneously with rs2294008CC, rs11599672AA and rs13361707TT genotype carriers (OR,6.792;95%CI,2.521～18.296).Conclusion:There may be an associton between PSCA (rs2294008), PLCE1(rs11599672), PRKAA1(rs13361707) gene polymorphism and alcohol drinking, family history of gastric cancer, H. pylori infection and gastric cancer in Gansu Han population, China.