Expression Of Gelsolin For Various Stages Of Primary Liver Cancer Generating Processes In Guangxi And Its Clinical Value Of Application

Objective To detect the expression of gelsolin in serum samples from highincidence area，to verify the mass spectrometry results of prophase research, toanalysis the relationship of GSN with AFP, gender, age, tumor diameter, tumornumber, etc. To discuss the joint detection value in diagnosis of PLC, and todiscuss the clinical value in PLC patients with AFP negative.Method ELISA and ECLIA were used to detect the GSN and AFP levels inserum specimen bank which was established by collecting serum samples fromhigh incidence area (PLC group30cases, liver cirrhosis group30cases, hepatitisB group30cases, normal control group30cases, hepatic benign occupyinglesions group30cases,5consecutive cases and postoperative group14cases,preoperative group14cases) and from Guangxi medical tumor specimens’library (other tumors group30cases). All data were analyzed with SPSS17.0.Results1. The concentration of GSN in PLC (1503.99±127.80pg/ml) is greater than that in liver cirrhosis (1317.63±124.80pg/ml), hepatitis B (1223.90±132.39pg/ml) and normal group (1037.13±116.81pg/ml),(P<0.05). Thisresult verifies the consistency of previous mass spectrometry, meanwhile thelevel of GSN in PLC is greater than that in hepatic benign occupying lesionsgroup (1116.45±146.42pg/ml) and other cancer group (1186.52±131.30pg/ml),(P<0.05).5consecutive cases reveal that concentration of GSN in PLC patientsis greater that liver cirrhosis or hepatitis B patients,(P<0.05).2. The concentration of GSN in postoperative group (1193.77±176.08pg/ml) islower than that in preoperative group (1492.34±127.61pg/ml),(P<0.05).3. There is no correlation between of expression of GSN and AFP in every group,(P<0.05).4. Draw the Receiver operating characteristic (ROC) curve. In diagnosis of PLCwith AFP, the AUC is0.706.The cut-off of AFP is21.58ng/ml, the sensitivityand specificity are53.8%,89.9%. The AUC of GSN in diagnosis of PLC is0.898%,(P<0.05). The cut-off of GSN is1414.42pg/ml, the sensitivity andspecificity are71.4%,87%. When two indicators combined, the diagnosticefficiency is increased distinctively with sensitivity86.7%and specificity90.4%.5. The expression of GSN in PLC patients is associated with the tumor diameter(P<0.05). But it isn’t associated with gender, age, tumor number, tumor emboliand AFP (P>0.05).Conclusion1.The mean concentration of GSN in PLC group is significantlygreater than those in liver cirrhosis group, hepatitis B group and normal group.Meanwhile it is greater than those in hepatic benign occupying lesions groupand other cancer group. This suggested that serum levels of GSN protein concentration is closely related to the occurrence and development of PLC. Thisresult verifies the consistency of previous mass spectrometry.2. The mean concentration of GSN in postoperative group is lower than that inpreoperative group. This provides a basis for subsequent research that GSN maybe used for prognosis in patients after hepatectomy primary liver cancer.3. The joint detection of GSN and AFP can improve the detection sensitivity ofPLC. GSN plays an important role in PLC patients with AFP negative, it can beused as a potential molecular maker of early diagnosis of PLC.4. The expression of GSN is associated with the tumor diameter.