Calcium Titanate Coating Fabricated On Titanium And Its Biological Performance

Objective To prepare the calcium titanate coating in the surface of pure titanium wasprepared through a simple hydrothermal synthesis method. To compare the effects ofthe calcium titanate coated and uncoated titanium on the osteoblast through theevaluation of biological properties. To explore the biological mechanisms of earlyosteogenesis on the interface of calcium titanate and bone, which provided theoreticaland experimental basis in clinical application of calcium titanate coating.Methods1, The titanium slices were placed in the reaction kettle with the solutioncombined with Tetra-n-butyl [(C4H9O)4Ti] and calcium hydroxide [Ca(OH)2],then theCaTiO3coating of titanium was prepared under conditions of different temperatures andtimes. Then the Microstructure, chemical composition and physical phase compositionof CaTiO3coating were detected.2, The biological safety of forming coating was assessed by bone marrow micro-nucleartest and the hemolysis test.3, Primary osteoblasts were cultured from the cranium of the SD rats by tissue blockculture, then they were purified and passaged. The maturity of osteoblasts was testedthrough the cell morphology, giemsa staining and calcified nodules staining. Theosteoblasts were cultured in the coating surface in vitro, the uncoated pure titanium wasprovided as control group. The state of cells spreading on the surface of the materialswas observed with microscope. The determination of MTT, relative protein content(Coomassie blue), alkaline phosphatase (ALP) activity of osteoblasts was measured byco-culture with materials and analysized statistically.4, Quantitative results were expressed as mean±SD. Chi-square test, T test, one-way analysis of variance (ANOVA) were performed with SPSS16.0software. P-valuesless than0.05were considered to be statistically significant.Results1, The CaTiO3coating of titanium could be prepared with the Hydroxyltitanium oxide produced by the hydrolysis of Tetra-n-butyl [(C4H9O)4Ti] and calciumhydroxide [Ca(OH)2] by the hydrothermal synthesis method. The temperature andthe time of the hydrothermal synthesis should be controlled. The main phase of coatingwas nano-calcium titanate.2, The results of Security toxicology experiments showed that bone marrowmicronucleus rate was under4‰and hemolysis experiments showed that the hemolysisrate was less than5%in vitro. The mutagenicity and hemolysis phenomenon were didnot show in the experiments.3, The observation of the morphology of primary osteoblasts and the positive results ofsubculture training osteoblasts dyeing experiments of giiemsa staining and calcifiednodules showed that the osteoblasts cultured in vitro had the morphological andbiological features which normal osteoblasts have.4, The cells proliferation and adhesive capacity were observed through testing theprotein content by MTT, coomassie brilliant blue method and alkaline phosphatase(ALP) activity. The results showed that the treated group had a increased compared withthe control group, the surface of titanium plate coated with calcium titanate was moreadvantageous to the adhesion and the proliferation on osteoblasts without toxic effects.Conclusions The titanium plates coated by calcium titanate had no significantmutagenic potential and hemolytic reaction. They enhanced the proliferation and thedifferentiation of the osteoblasts. Calcium titanate is a kind of promising coatingmaterial with potential development capability which provide experimental basis for asimple and effective preparation of the coating material and the clinical application of biological materials.