Inhibitory Effects Of Evodiamine Combined With Radiotherapy On The Growth Of Xenotransplants Of Human Tongue Squamous Cell Tca-8113Cells In Nude Mice

Tongue squamous cell carcinoma is a high incidence disease in oral cancers. Surgery and radiotherapy are the main methods for the treatment of tongue cancer. However, the effect of radiotherapy is limited owing to the injury of healthy tissue and the resistance of tumor tissue. In clinical therapy, the use of radiosensitizer can improve the radiation effect on tumor cells. However, ideal radiosensitizer is nonexistence. Thus, Choose a good radiation sensitizer treatment has become a new way to optimize therapy of tongue cancer.To investigate the effects of EVO in combination with radiation on the growth of xenotransplants of human tongue squamous-cell Tca-8113cells in nude mice. Sixteen nude mice bearing subcutaneous xenotransplants of tongue squamous-cell cancer Tca-8113cells were randomLy divided into four groups:control group (C, no treatment), EVO-treated group (E), radiation-treated group (R), and EVO in combination with radiation-treated group (E+R), corresponding drugs and radiation treatments were given respectively. These nude mice were observed for28d. Tumor growth curve was drawn and tumor inhibitory rate was calculated. HE staining was used to observe the change of cells in morphology. The immunohistochemical method was used to detect Ki67, Bax and Bcl-2expression in tumor cells. The ultrastructure of apoptotic cells were observed by transmission electron microscope (TEM).The tumor volume was smaller in group E+R than that in any other groups from the8th day after treatment (P<0.05). On the28th day after treatment, the tumor inhibitory rates of group E, group R, and group E+R were15.15%,43.29and63.30%, respectively. A great amount of cell necrosis was observed in group E+R by HE staining. Immunohistochemistry detection showed that in group E+R, the expression of Bax increased while the expression of Bcl-2and Ki67decreased. In group E+R, the characteristics of cellular apoptosis were observed.Conclusion:EVO in combination with radiation could inhibit the growth of xenotransplants of human tongue squamous-cell Tca-8113cells in nude mice, this effect may be associated with down-regulation of Ki67and Bcl-2expressions, and up-regulation of Bax expression.