Serum Proteomics Research For The Rabbit Articular Cartilage Early Damage

Objective:Two-dimensional gel electrophoresis was used to isolate serumprotein. The differential protein spots were identified by matrix assisted laseranalytical time of flight mass spectrometry(MALDI-TOF-MS).To analyze theproteomic components from the the normal cartilage and damage cartilage,andto search proteins that might serve as serum biomarkers for bone marrowmesenchymal stem cell migration of repair cartilage damage.Methods:Preparing20healthy adult New Zealand white rabbits andrandomly divided into damage group and the control group.Drilling a4mm indiameter and depth of about3mm hole in front of the lateral femoral,causing afull-thickness articular cartilage damage model. Collected serum samples of thecontrol group and damaged group after two and four weeks. Two-dimensionalgel electrophoresis was used to isolate serum protein. The differential proteinspots were identified by matrix assisted laser analytical time of flight massspectrometry(MALDI-TOF-MS).To analyze the proteomic components fromthe the normal cartilage and damage cartilage.Results:By removing the serum high abundance proteins and optimize thetwo-dimensional gel electrophoresis.We get the maps of background clear, high resolution and good repeatability and found20different proteins in theimage.Seven proteins were successfully identified by mass spectrometryanalysis. They were haptoglobin, complement factor B, complement C1q,cluster precursor protein, beta globin, and alpha1protease precursor E.Conclusion:Using serum proteomics techniques we got good two-dimensional gelelectrophoresis maps and found different proteins between the damage articular cartilageand the normal articular cartilage serum.These proteins might serve as serum biomarkersfor bone marrow mesenchymal stem cell migration of repair cartilage damage.