Analysis Of The Syndrome About Pulmonary Interstitial Lung Disease Patients And The Intervention Of Ginseng Seven Cordyceps Capsule On Effects Of MMP-9and TIMP-1in Pulmonary Fibrosis Rats Cause By Bleomycin

Objective:1In order to explore the TCM syndrome distribution law of pulmonary interstitialfibrosis and provide theoretical basis for clinical practice, through the clinicalsymptomatology survey based on investigation of82patients with syndrome.2To explore Shenqi-Chongcao capsule on the mechanism of pulmonary fibrosisthrough observing Shenqi-Chongcao capsule on effects of matrix metalloproteinases-9and Tissue inhibitor of metalloproteinases-1in Pulmonary Fibrosis Rats.Methods:1.Investigation of clinical syndromes: In access to relevant literature, combined withclinical experience and the direction of the superior doctor, I developed pulmonaryinterstitial fibrosis syndromes questionnaire for TCM syndrome investigation on82cases of patients with pulmonary fibrosis and collected82cases of this disease ingeneral, inducing factors, four diagnostic methods of TCM information, to investigatepulmonary interstitial fibrosis of the distribution of TCM syndrome by analyzing therelated factors.2. Experimental study on animals:80SD male rats were divided into four groupsrandomly including control group,model group,prednisone group and Shenqi-Chongcao group,20in each group. Each rat freely drank water.Adaptive feeding oneweek later,except the normal control group,the other groups were all adoptedbleomycin(BLM)to set up pulmonary fibrosis rat model and filled the stomach everyday. the contents of MMP-9and TIMP-1of lung tissue were detected by HE dyeing,immunohistochemical staining method and the pathological changes of lung tissueafter15and30days.Result：1Among82patients of the investigation in accordance with the standard, there are8cases with syndrome of deficiency of Qi and exogenous wind-cold,accounting for9.76%;5cases with syndrome of deficiency of jin and phlegm-heat type, accounting for6.10%;7cases with syndrome of deficiency of Qi in Lung and spleen, accountingfor8.54%;31cases with syndrome of Yin deficiency with phlegm and blood stasissyndrome, accounting for37.80%;17cases with syndrome of Yin scorching lunginjury, accounting for20.73%;4cases with syndrome of the phlegm and blood stasis,accounting for4.88%.7cases with syndrome of the phlegm heat in lung, accountingfor8.54%.3cases with syndrome of the phlegm wet aggregates lung, accounting for3.66%.2Comparison of82ILD patients and46healthy human blood examination indexed:Prothrombin time of ILD patients in group are higher than that of the healthy groupand the difference was statistically significant (P<0.01); IgG, IgM,IgA levels of ILDpatients in group were higher than that of the healthy group and the difference wasstatistically significant (P<0.05); SOD levels of ILD patients in group were lowerthan that of the healthy group and the difference was statistically significant (P<0.05);LDH levels of ILD patients in group was higher than that of the healthy group and thedifference was statistically significant (P<0.01); CRP, ESR levels of ILD patients ingroup was higher than that of the healthy group and the difference was statisticallysignificant (P<0.01).3Lung tissues were observed under the light microscope in animal experiment. Thealveolar cavity refreshing no infiltration without damage of structure in the blankgroup. The main macrophages and lymphocytes on points of a large number ofinflammatory cells were infiltrated, accompanied by alveolar septum broadening andbronchus near fibrosis was obvious in the model group on15days.The alveolitis andpulmonary fibrosis on points of30days were more serious than the15days. Therewere large crumb macrophages, lymphocytes and inflammatory cells infiltration,accompanied with damage of lung tissue structure, narrow alveolar space, pulmonaryinterstitial collagen fibers,fibroblasts and pulmonary fibrosis formation on30days. InShenqi-Chongcao capsule and prednisone group on15days the main changes weremild to moderate alveolitis fibrosis accompanied with the infiltration of inflammatorycells,and on30days point the degree of pulmonary fibrosis increased, but lighter than the model group.Immunohistochemical results showed: The expression of blank group of MMP-9and TIMP-1were weak in the process of the whole experiment and the proteinexpression of the model group rats of MMP-9and TIMP-1each time weresignificantly higher than control group during this period and there was a verysignificant difference compared with the blank group (P<0.01). With the passage oftime, the expression of protein on MMP-9in30days was on the decline, but stillhigher than the blank group. Expression of TIMP-1continued to increase, reachingthe highest on30days compared with the control group and there was a verysignificant difference (P<0.01). Compared with the model goup the expression ofMMP-9and TIMP–1in Shenqi-Chongcao capsule and prednisone group decreasedeach period and there was a significant difference or extremely significant difference(P<0.05or P<0.01), but no obvious difference between two groups(P>0.05).Conclusion:1From pulmonary interstitial fibrosis we could find that the syndrome of deficiencyof Qi and Yin with phlegm was the common syndrome of pulmonary interstitialfibrosis.2Shenqi-Chongcao capsule could decrease the alveolitis and pulmonary fibrosis andthe mechanism of that could decrease the expressions of MMP-9and TIMP-1in lungtissue and reestablish the balance between MMP-9and TIMP-1to inhibit theformation and development of pulmonary Fibrosis Rats.