Mao Lily Tissue Culture And Research Bulb Inflated Tube

In this experiment, using wool scales, aseptic seedling leaf as explants, the regeneration system of tissue culture was established, which lays the theoretical foundation for breeding, genetic transformation, garden application and production of Lilium dauricum. Study the effect of different concentration of salicylic acid (SA), NAA, paclobutrazol(PP333), macroelement, sucrose, activated carbon(AC), and medium state on growth and formation of bulb, providing basis theory and key technology for domestication and vitro micro-bulbs factory production of Lilium dauricum.1Use Lilium dauricum bulb as explants to establish regeneration system of tissue culture.It was showed that the sterilization with75%alcohol and2%sodium hypochlorite sterilizing12min was optimal; intermediate bulb was the best explant for tissue culture, the ability of induction and differentiation of different parts from strong to weak was base, middle, apically; the single factor experiment showed that6-BA had a direct relationship with adventitious bud inducing and callus formating, the optimal medium for scale inducing adventitious buds was MS+6-BA2.0mg/L, differentiation rate was86.7%, differentiation coefficient was4.70; the optimum medium for induction and differentiation of callus was MS+6-BA (1.0-2.0mg/L), induction rate was83.3%, differentiation coefficient was6.25;2,4-D (less than2.0mg/L) promoted the formation and differentiation of callus, but had no effect on differentiation of adventitious buds; the double factors test showed that the best medium for induction and differentiation of callus was MS+6-BA0.5mg/L+2,4-D1.0mg/L; induction rate was90%, differentiation coefficient was7.44; the optimum medium for adventitious buds induction was MS+6-BA0.5mg/L+NAA0.5mg/L, differentiation rate was86.7%, differentiation coefficient5.69; the optimum medium for bud multiplication was MS+BA1.0mg/L+NAA0.1mg/L, multiplication coefficient was7.8. The best rooting medium was MS+AC0.5mg/L, the rooting rate was100%, survival rate was100%when transplanting to pearlite matrix.2. The regeneration system was established with aseptic seedling leaf as explantExperiment showed that the differentiation of leaf had significant position effect, ability of differentiation of leaf from strong to weak was base, middle, apically; the optimum medium for adventitious buds differentiation was MS+NAA0.3mg/L+6-BA2.0mg/L, differentiation rate was86.7%, differentiation coefficient was4.31; the optimum medium for adventitious bud multiplication was MS+NAA0.1mg/L+6-BA2.0mg/L, multiplication coefficient was4.93, and growing better; the best rooting medium was1/2MS+NAA1.0mg/L and1/2MS+IBA1.0mg/L, the rooting rate was100%, seedling survival rate was up to82%.3Research of bulb swelling Experiment showed that when AC concentration was1.0g/L, bulb diameter increased2.50times, fresh weight was828.2mg, node bulb rate100%, and the increased bulb number was4.40; when the NAA concentration was1.0mg/L, bulb diameter increased2.26times, fresh weight was658.4mg, the increased bulb number was2.60; when the IBA concentration was ranged from1.0to2.0mg/L, node bulblet rate was100%, bulb diameter increased2.04times, when the concentration of IBA was1.0mg/L, the increased bulb number was2.57, when the IBA concentration was2mg/L, it was more propitious to the expansion of bulbs, fresh weight was675.8mg;2MS had the best effect on the formation and enlargement of the bulb, bulb diameter increased2.37times, fresh weight was696.5mg, node bulb rate100%, and the increased bulb number was3.60; when the PP333concentration was10mg/L, bulb diameter increased2.20times, bulb fresh weight was625.4mg, and node bulb rate was100%. Salicylic acid was available to improve the induction rate of bulb, but not conducive to bulb expansion. When salicylic acid concentration was0.5mg/L, node bulb rate was87%, the increased bulb number was4.63. Considering the inoculation convenience and bulb swelling effect, half solid culture method was prefect, fresh weight was448.2mg, node bulb rate92%, average bulb2.70; small bulbs with enlargement processing can be transplanted without rooting, whe the bulb diameter was greater than8mm,the plant growth was better and easy to survive after transplantation.