Studies On Formation And Enlargement Of Lily (lilium L.) Bulblets In Vitro

The sterile seedlings of Lilium,longiflorum,Lilium.asiatic hybrids 'Acuba','Nove Cento' and Lilium.lancifolium were taken as explants to induce the bulblets in vitro.In order to improve factory production of bulblets from sterile seedlings,the tissue culture condition of the technique of bulblets enlargement was optimized.1.Filament and pedicel of L.longiflorum were taken as explants to induce plantlet. The medium of MS+6-BA0.5mg/L+NAA0.1 mg/L and MS+6-BA1.0mg/L+NAA0.5mg/L were suitable to induce the callus and differentiation for the filament and pedice respectively;The medium of MS+6-BA1.0mg/L+NAA0.1 mg/1L showed the best effect for the buds multiplication.2.Effect of culture condition on the bulblets enlargement Within a certain range, raising the concentration of sucrose could increase the diameter of bulblets.But the excessive high concentration(120g/L) could weaken the effect.The concentration of 90g/L was the optimal concentration for the culture of bulblets in vitro,either L.longiflorum or 'Acuba',the fresh weight of bulblets came up to 107.86mg and 127.97mg respectively.The medium additioned 5mg/L PP₃₃₃ showed the best fresh weight of L.longiflorum bulblets in vitro,it was 99.22mg;for 'Nove Cento',the concentration of 10mg/L was suitable.The concentration of 0.5mg/L NAA showed the best effect to increasing the fresh weight of L.longiflorum bulblets,it came up to 122.64mg;the concentration 2.0mg/L NAA were suitable for the enlargement of 'Nove Cento' bulblets.In the experiment of orthogonal design,the medium with 90g/Lsucrose,10mg/L PP₃₃₃ and 0.025mmol/L SA was suitable for 'Acuba' bulblets enlargement;the medium with 90g/L sucrose,1mg/L PP₃₃₃ and 0.1mmol/L SA was suitable for 'Nove Cento'.AC had powerful effect on promoting leaf growth and rooting,and had powful effect on promoting bulblet enlargement.3g/L AC was suitable for bulblets culture of L. longiflorurn and L.lancifolium,and the fresh weight of them was 2～3 times to CK.Me-JA had powerful effect on L.longiflorum,it made the fresh weight up to 110.77mg;but there was no notable difference on L.lancifolium.The shallow liquid system was useful for the bulblets to absorbe the nutrition,so it was good for the diameter increasing.There was best fresh weight on the shallow liquid system for L.longiflorum and L.lancifolium,it came up to 161.62mg and 249.84mg respectively,and the percentage of large bulblets(dm＞8mm) was 48.53%and 54.05% respectively.This culture system could also shorten the time of bulblets culture to 60 days. The liquid culture containing sucrose and AC simultaneity had more powerful effect on promoting leaf growth,rooting and bulblet enlargement than the effect of using sucrose or AC respectively.The medium with 60g/L sucrose and 3g/L AC showed the best effect to increasing the fresh weight of L.longiflorum bulblets,it came up to 627.32mg, and the percentage of bulblets(dm＞13mm)was up to 39.02%.The medium with 90g/L sucrose and 3g/L AC was suitable for the culture of L.lancifolium bulblets enlargement, the fresh weight of bulblets came up to 479.17mg,and the percentage of bulblets (dm＞13mm) was 10.25%.3.The bulblets from the solid medium were classified to three grades on the basis of diameter A:＞8mm,B:5-8mm and C:3-5mm.The living rate of L.longiflorum and 'Nove Cento' were respectively 95.8%,81.9%,80.6%and 85.7%,68.3%,44.4%;After sowing, the living seedlings from bigger diameter became stronger.The bulblets from the liquid medium were classified to three grades on the basis of diameter D:＞12mm,E:9-12mm and F:7-9mm.The living rate of L.lancifolium in different grade were up all to 85%.The bulblets whose diameter up to 12mm grew best,and the diameter was over 7mm after planting for 60days.