White Wheat TaAmy3 Gene Allelic Variation And Correlation Analysis Sprouting Resistant

Pre-harvest sprouting would seriously decrease yield and quality of wheat, resulting in economic losses. Alpha-amylase activity is the most important factor influencing pre-harvest sprouting of wheat. It is very important to develop resistant varieties as the effective method by molecular-assisted breeding via utilizing alpha-amylase genes. The allelic variations of TaAmy3genes from the white wheat accessions collected worldwide and the associations between TaAMY3genes and the resistance to pre-harvest sprouting were analyzed.. The main findings of this study are as follows:1. The pre-harvest sprouting of163wheat accessions was identified in three places for two years. Four strongly resistant and15resistant accessions were selected from the identification results of2011in Ya’an, and5resistant accessions were selected from the identification results of2012in Ya’an. Six strongly resistant and5resistant accessions were selected from the identification results of2012in Wenjiang. Two strongly resistant and4resistant accessions were selected from the identification results of2012in Dujiangyan. Through the identification results at three places for two years,7strongly and stably resistant wheat accessions (i.e. AS660608, AS660571, AS660578, AS660583, AS660579, AS660272and AS660365) were selected.2. The analysis of TaAmy3gene sequences of among163wheat accessions indicated that TaAmy3gene was very conservative, and36SNPs including two insertion/deletion-bit point, with an average of one SNP per40.28bases, were identified, among which27SNPs occur in exon, and the rest exist in two introns. In the deduced amino acid sequences, there were401amino acids residues, and the SNPs in coding region result in10amino acids mutants.163wheat accessions were divided into17haplotypes according to TaAmy3gene sequences, wherein13haplotypes contained only one accession. The genetic cluster analysis of TaAmy3genes among the163wheat accessions showed that TaAmy3genes were not associated with seed coat color or geographical distribution of wheat accessions, indicating that the variation of TaAmy3genes was little related to seed coat color and geographical distribution.