Development And Validation The Pre-harvest Sprouting Tolerance And Identification The GA20ox1Allelic Variations In Chinese Historical Wheat

Historical wheat in China was not only rich genetic resources, but also are parental resources in wheat breeding. At present,the Pre-harvest-sprouting (PHS) in wheat is a serious problem in safe of wheat production and improving the quality of wheat, therefore, breeding PHS tolerance wheat has an urgent problem in wheat breeding. Marker-assisted selection in wheat breeding can shorten the time of breeding effectively. We selected Vp1A3and Vp1B3associated with PHS tolerance to evaluate PHS tolerance varieties in a set of historical wheat germplasm in Chinese. The results indicated that, Eight alleles of Vp1-A were detected, which were Vp1-Aα, Vp1-Ab, Vp1-Ag, Vp1-Aj, Vp1-Ak, Vp1-Ai, Vp1-Ah and Vp1-Al, with frequencies of28.04%,8.41%,2.80%,7.48%,3.74%,38.32%,8.41%and1.87%, respectively. In addition, the Anxuan2was the new type, named Vpl-Ar, compared with Vp1-Aα, which had a15bp (5CTT) deletion in position of2718bp-2732bp. Three alleles, which were Vp1-Bc, Vp1-Ba and Vp1-Bb, were detected by marker Vp1B3associated with PHS tolerance with proportions of44.86%,44.86%and9.35%, respectively. Combination with the result of Vp1A3, Vp1B3and germination index, five resistance genotypes were selected out, which were Anxuan2, Yannong15, Zhengzhou683, Zhengzhou742and Kequn. The haplotypes of Vp-1in these five cultivars were Vp1-Ar and Vp1-Bc; Vp1-Ah and Vp1-Bc; Vp1-Ab and Vp1-Bc; Vp1-Ag and Vp1-Bc; Vp1-Ah and Vp1-Bc.We also research the allelic variation in Ga20oxl in different PHS tolerance wheat. Compared with the sequence of Y14008, Ga20oxl-B of Zhongyou9507and Jingdong1had three SNPs, which all lead the change of amino acid; For GA20oxl-D, Jingdong1had three SNPs at the position of379bp,581bp and1101bp, respectively, and Wanxianbaimaizi had a SNP at positions of1161bp, these SNPs all lead the change of amino acid.