Experimental Study On Anti-fatigue Effect Of Rutin

The article summarizes the molecular formula, structure, source of rutin and its extraction and separation methods, its pharmacological effects and clinical application; and also generation mechanism of fatigue and its relationship with free radicals and lipid peroxidation. Through determining the best dose of rutin on fatigue in mice, this paper would do some further in-depth studies of the anti-fatigue mechanism of rutin.Also known as rutoside, rutin is a kind of flavonoids. It is separated from plants which widely exist in nature, and almost all of the rutaceae and heather contains rutin. The study shows that rutin has many pharmacological functions such as resistance to free radicals and lipid peroxidation, anti-aging, antibacterial, lowering blood pressure, anti-uv protection and so on.This experiment includes two parts. To investigate the effect of rutin on fatigue resistance and to provide scientific evidence for the development of anti-fatigue drug, swimming-induced injury model was adopted to investigate the optimal dose of rutin on fatigue resistance in mice. The mice in rutin-supplemented group were intragastric administrated with different doses of Icariin. The mice in control group were intragastric administrated with the same volume of sodium carboxymethylcellulose for12days. After exhausted swimming and recovering for20hours, the mice were executed. The alanine aminotransferase(ALT), asparteta transaminase(AST), malonaldehyde/malondialdehyde (MDA), blood urea nitrogen (BUN) and serum glucose (SG) in serum were determined by ultramicro micro hole plate spectrophotometer, and the isozymes of lactate dehydrogenase (LDH) were detected with PAGE. Then, with this exhaustive swimming-induced fatigue model, the ALT, AST, MDA, BUN and SG in blood serum, superoxide dismutase (SOD), MDA and glycogen in liver, SOD, MDA and glycogen in muscle, SOD, MDA in heart, SOD, MDA in brain were detected. Moreover, the electro-phoresis of LDH in blood serum, liver, muscle, heart and brain were analysed to explore the mechanism of physical performance promotion.Experimental results of the first part:1. Compared with the control group, the endurance time of exhausted swimming was apparently lengthened in the mice of rutin middle-dose group.2. Compared with the control group, the activities of ALT and AST, the contents of MDA and BUN, and the total activity of LDH isozymes were decreased significantly in the mice of rutin middle-dose group, and could effectively help SG levels return to normal, indicating that the middle-dose of rutin has fairly strong function on anti-fatigue.Experimental results of the second part:1. Rutin could obviously prolong the endurance time of exhausted swimming, and the ratio was20.30%.2. Under the quiet state, rutin had no obvious effects on MDA, AST, ALT, BUN, SG in mice serum. However, it could significantly reduce the contents of MDA, BUN and AST, ALT activity in exhaustion exercise mice so as to play a protective effect on organism. At the same time rutin could effectively remove MDA, BUN in serum and could significantly improve the recovery process of mice blood sugar levels to normal after exhaustion in mice.3. Rutin could improve the SOD activity in liver, muscle, heart and brain of exhaustive exercise mice, and could reduce the content of MDA in the recovery condition, and increase the reserves of liver glycogen and muscle glycogen in the quiet state, and could effectively prevent the exhaustion of liver glycogen and muscle glycogen in the process of exhaustion, which could be the important mechanism of rutin to prolong the anti-fatigue endurance time.4. The results of serum, brain, heart, quadriceps, liver electrophoresis clearly showed that in the exhaustion sports and the recovery after exhaustion, LDH4-5, LDH, LDH1-2were conducive to enhance the mice movement changes.Above all, rutin can relieve the oxidative damage of organs, and regulate the body’s metabolic state, to improve the sports ability of mice, and promote rapid recovery from fatigue status in mice. Development and utilization of rutin whether can be used as the anti-fatigue drugs, deserves further research.In conclusion, rutin can relieve the oxidative damage of organs, and regulate the body’s metabolic state to improve the sports ability of mice, and to promote mice to recover from fatigue status rapidly. Whether rutin can be exploited to be the anti-fatigue drugs deserves further research.