Comparative Study Of Rabbit Knee Osteoarthritis, Lateral Collateral Ligament Mechanical Impact On The Knife And Needle Intervention

Osteoarthritis (OA) is a clinical common and frequently-occurring disease. Data show that: the prevalence of the crowd of60years old and above reached50%, and75year olds can be as high as80%. The disability rate of OA is as high as53%. O A most commonly involves the more active and bearing knee joint. The knee osteoarthritis (KOA) research is the focus of scholars at home and abroad. Acupotomy treatment of KOA has certain advantages in clinical practice, and have made some progress on basic research. But the research about ligament biomechanics is still weak, and the research about Acupotomy therapy compared with traditional acupuncture therapy for knee osteoarthritis is extremely rare. Under the guidance of Chinese medicine theory, referring to surgery operation principle of western medicine and combined with their own understanding of acupotomology on knee osteoarthritis, this study tries to explore the effect of acupotomy therapy and acupuncture therapy on ligament mechanical changes and the relationship between the two.ObjectiveIn a multi-centric clinical study of RCT of knee osteoarthritis in early, it has proved that it has significant effect to treat the disease by acupotomy. The rabbit model of KOA was established by immobilizing the left hind limb in extension position. This research used acupotomy and acupuncture to intervene the KOA rabbits respectively and observe the effect of two kinds of intervention methods on mechanical properties of medial and lateral collateral ligaments in these KOA rabbits, to explore the law of acupotomy and acupuncture intervention effect on the mechanical changes of ligament and the relationship between them, to clear the important function of the ligaments in the Jingjin of knee, to reveal the scientific connotation of Jingjin "bunch of bone and joint" in Jingjin theory and mechanism of acupotomy recovering joint mechanics balance by correction "Jin" and" bonesetting ", and to lay a good foundation for further study of plausible mechanisms of acupotomy intervention of KOA.MethodsThe48June age New Zealand rabbits, half male and female, weight2.25+0.25kg, were randomly divided into four groups, with12in each group, four groups were blank group, model group, acupotomy group and acupuncture group. The rabbit model of KOA was established by immobilizing the left hind limb in extension position. After braked for6weeks, 6rabbits of each group were randomly selected to be observed the behavior changes. When behavior results suggested the success of modeling, all animal external plaster were removed, and then into the treatment intervention on acupotomy group and acupuncture group. We took a behavioral observation to each group in the one week after the end of treatment. After drawing the experiment materials, we took the tensile, creep and stress relaxation tests of internal and lateral collateral ligament at the Bose Electro Force3300fatigue test machine. Finally, the data were statistically analyzed.Result1Behavioral observations:Lequesne MG score display that before treatment, model group, acupotomy group and acupuncture group are no difference (P>0.05), and show significant difference from normal group (P<0.01), suggesting that fixed after6weeks, compared with normal group, the pain, gait, motion and swelling behavior of ill joint of the model group has obvious changes, which shows that the experimental model is successful. Before and after treatment, the scoring differences of acupotomy group and acupuncture group are significantly different from model group (P<0.01), and the difference of acupotomy group is slightly larger than acupuncture group. Acupotomy and acupuncture intervention can effectively improve pain, swelling, gait, and activity behavior of joint of KOA limb.2The tensile test results of medial collateral ligament:1) the maximum stress:compared with the normal group, model group, acupotomy group and acupuncture group are significantly lower than normal group (P<0.01); acupotomy group is significantly higher than the model group (P<0.01); acupuncture group is less than the acupotomy group (P<0.05) but has no difference from the model group (P>0.05).2) the maximum strain:the model group is less than the normal group (P<0.05), and there is no difference between acupotomy group, acupuncture group and the normal group (P<0.05); compared with the model group, the maximum strain of acupotomy group is bigger (P<0.05), the acupotomy group is much more bigger (P<0.01), and the difference between acupotomy group and acupuncture group is not significant (P>0.05).3) the maximum displacement: the model group is smaller than the normal group (P<0.05), and acupotomy group, acupuncture group compared with the normal group has no difference (P>0.05); acupuncture group is significantly higher than the model group (P<0.01), and larger than the acupotomy group (P<0.05).4) the elastic modulus:compared with the normal group, model group, acupotomy group and acupuncture group are significantly lower than normal group(P<0.01); acupotomy group is bigger than model group (P<0.05); acupuncture group is significantly less than the acupotomy group (P<0.05) but has no difference with the model group (P>0.05). It is not difficult to see, the data of model group is smaller than that of normal group (P<0.05, P<0.01);all tensile index of acupotomy group are higher than the model group(P<0.05, P<0.01) except the maximum displacement (P>0.05); the maximum stress and elastic modulus of acupotomy group is higher than acupuncture group(P<0.05, P<0.01);the maximum displacement of acupotomy group is significantly lower than acupuncture acupotomy group (P<0.01); the maximum strain of acupotomy group compared with the acupuncture group has no difference (P>0.05); the maximum stress and elastic modulus of acupuncture group has no difference with the model group (P>0.05), and the maximum strain and maximum displacement is significantly greater than that of the model group (P<0.01).3The stress relaxation rate and creep rate of medial collateral ligament:the stress relaxation rate and creep rate between the four groups is significantly different (P<0.01). The pairwise comparison of the stress relaxation rate shows that model group, acupotomy group and acupuncture group are significantly higher than those in the normal group(P<0.01); acupotomy group and acupuncture group are significantly lower than model group (P<0.01); acupuncture group is significantly higher than acupotomy group (P<0.01). The pairwise comparison of the creep rate shows that model group, acupotomy group and acupuncture group are significantly lower than those in the normal group(P<0.01); acupotomy group and acupuncture group are higher than model group (P<0.01, P<0.05); acupuncture group is significantly lower than acupotomy group (P<0.01).The results show that the rate of stress relaxation and creep rate of the medial collateral ligament has changed, and the acupotomy and acupuncture intervention can both effectively restore the stress relaxation and creep properties, and recovery ability of acupotomy treatment is better than that of acupuncture treatment.4The tensile test results of lateral collateral ligament:1) the maximum stress:compared with the normal group, model group, acupotomy group and acupuncture group are significantly lower than normal group (P<0.01); acupotomy group and acupuncture group are significantly higher than the model group (P<0.01); acupuncture group is less than the acupotomy group (P<0.05).2) the maximum strain:compared with the normal group, there is no difference between model group, acupotomy group, acupuncture group and the normal group (P>0.05); acupotomy group and acupuncture group has no difference with the model group (P>0.05); acupuncture group is bigger than the acupotomy group (P<0.05).3) the maximum displacement: compared with the normal group, model group, acupotomy group and acupuncture group have no difference with the normal group(P>0.05); acupotomy group, acupuncture group compared with the model group have no difference (P>0.05), acupuncture group is significantly higher than acupotomy group (P<0.01).5The stress relaxation rate and creep rate of lateral collateral ligament: the stress relaxation rate and creep rate between the four groups is significantly different (P<0.01). The pairwise comparison of the stress relaxation rate shows that model group, acupotomy group and acupuncture group are significantly higher than those in the normal group(P<0.01); acupotomy group and acupuncture group are significantly lower than model group (P<0.01); acupuncture group is significantly higher than acupotomy group (P<0.01). The pairwise comparison of the creep rate shows that model group, acupotomy group and acupuncture group are significantly lower than those in the normal group(P<0.01); acupotomy group and acupuncture group are higher than model group (P<0.01, P<0.05); acupuncture group is significantly lower than acupotomy group (P<0.01).The results show that the rate of stress relaxation and creep rate of the lateral collateral ligament has changed, and the acupotomy and acupuncture intervention can both effectively restore the stress relaxation and creep properties, and recovery ability of acupotomy treatment is better than that of acupuncture treatment.ConclusionAcupotomy and acupuncture with the same diameter intervene the same needle points to treat knee osteoarthritis model can effectively improve the tensile, relaxation and creep mechanical properties of knee osteoarthritis model rabbits, and the improvement of acupotomy is better than acupuncture.