| Knee osteoarthritis with age, the incidence of knee osteoarthritis is g adually increased. Clinical manifestations is joint pain, tenderness, join swelling, limited mobility and joint deformity. Clinical application of acu otomy on knee osteoarthritis has a good effect. However, the mechanical asp cts of its mechanism and action is no in-depth study. This study is part o relying China natural science foundation, acupotomy on knee osteoarthritis igament mechanics changed and chondrocytes mechanical signal transduction a feet. The experimental study is corresponding basic research from soft tiss e angle. Clarify of acupotomy for osteoarthritis rabbit knee ligament tissu the mechanical properties adjustments and effectively regulate ligamen Collagen associated factor gene and protein. For the clinical treatment of nee osteoarthritis provide new ideas and methods.ObjectiveBy acupotomy "Method of adjustment tendons and treatment bone" treatmen koa. Observation acupotomy to koa rabbit model ligament mechanics change, nd acupotomy to impact on ligament Collagen associated factor gene protein xpression. Authentication treatment koa by acupotomy loose on ligament adhe ions, adjustment ligament mechanical balance, regulate ligament TGF-β1, CO-I and MMP-13gene protein expression, restore ligament structure and funct on, conducive to the stability of the knee, reducing the noxious stimuli of cartilage, to inhibit the change of cartilage matrix, restore knee mechanic balance this hypothesis, reveals the scientific connotation of Basic Theor of traditional chinese medicine, acupotomy Basic research open a new chaptMethodsSix-month-old healthy New Zealand rabbits50(equal between genders) wer randomly divided into normal control group, model group, acupotomy group, lectro-acupuncture group, we selected immobilized left knee joints of rabbi s for koa rabbit models. When one week after the end of modeling, we starte treating acupotomy and electro-acupuncture. Treatment before and treatment fter were observed in Each group rabbits behavioral and imaging manifestat ions; observed the pathology of the joint cartilage and Ligament through op ical microscope and electron microscope; We also tested the tension, creep a nd stress relaxation data of lateral collateral ligament; Real-time PCR and Western blot detection TGF-β1, COL-I, MMP-13, TIMP-1mRNA and protein expres sion levels. Respectively a comparative analysis of these indicators, observ ation acupotomy treatment regulation of koa rabbits.Results1Behavioral results:Before treatment, Model group, acupotomy group and electro-acupuncture group Lequesne MG score statistics no difference (p>0.05), After treatment, Three group there are different degrees of change. Whic h acupotomy Group the most significant before and after treatment scoring di fference (p<0.01). Acupotomy treatment can improve the koa rabbits arthralgi a, activity, swelling and gait of joint.2Imaging results:Before treatment, the model group, acupotomy group an d electro-acupuncture group X-ray showed the koa rabbit knee joint space nar rowing, joint surface deformation, articular surface is uneven, the tibial p lateau bone hyperplasia, subchondral bone density uneven. After treatment, t he acupotomy group and electro-acupuncture group had improved to varying deg rees, Rabbit knee had slightly widened joint space, bone hyperplasia been re duced.3Tissue morphology results:Observed by light microscopy and electron m icroscopy showed, After modeling, tissue inflammation Significant,After trea tment, the acupotomy group and electro-acupuncture group had improved to var ying degrees, but acupotomy group effectively improve better than electro-ac upuncture group. Acupotomy treatment on osteoarthritis rabbits knee reduces tissue inflammation. Reduce the abnormal proliferation of cells, reduce the abnormal changes of collagen fibers, reduce matrix degeneration, promote tis sue repair. Acupotomy treatment regulating for cartilage and ligament tissue.4Biomechanics Results:After modeling, Model group compared with the no rmal control group lateral collateral ligament relaxation, creep and tensile mechanical properties showed remarkable differences. After treatment, the a cupotomy group and electro-acupuncture group had varying degrees of change, both groups were can improve the mechanical properties, acupotomy on the rel axation rate had a positive impact, compared with electro-acupuncture group improved significantly, close to the level of the normal control group. 5Real-time PCR and Western blot Test results:After modeling, Knee oste oarthritis rabbits lateral collateral ligament TGF-β1, COL-I, TIMP-1mRNA an d reduced levels of protein expression, MMP-13expression level increased. S tatistical differences compared with the normal control group. After treatme nt, the acupotomy group and electro-acupuncture group has a regulatory role, can TGF-β1, COL-I, TIMP-1mRNA and protein expression level increased, decr eased expression levels of MMP-13. Prompt acupotomy or electro-acupuncture t reatment for ligament TGF-β1, COL-I, TIMP-1mRNA and protein expression has played a good role in regulating, but acupotomy regulation is better than e lectro-acupuncture.Conclusion1Acupotomy treatment can improve knee osteoarthritis rabbit tissue infl ammation, reduce matrix degeneration and promote tissue repair. Acupotomy tr eatment on cartilage and ligament tissue has a good repair.2Acupotomy treatment effectively adjust the biomechanical properties of ligaments, restore ligament can be affected by stress, regulating ligament function, restore knee mechanics balance.3Acupotomy treatment effectively regulate ligament matrix, restoring th e structure and function of ligament, inhibit the pathological process of kn ee osteoarthritis.4Acupotomy treatment for knee joint mechanics adjustment effect, may be an important mechanism for the treatment of knee osteoarthritis.
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