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Cortex Phellodendri Amurensis is the dry cortex of Phellodendron amurense Rupr.. As it is made from northeast of China, it is called guan cortex phellodendri.It has a long history of medicine, and it has the actions to clear away heat. In the paper, based on experimental results, active constituents were found out and the quality criterion was established. By comparing Phellodendron amurense Rupr. and P. chinensis Schneid., the qualitative and quantitative identifications were found out, offering more necessary material foundations for drugs further research.1. The development of cortex phellodendri amurensis was summed up, providing a reference for further research and development of cortex phellodendri amurensis.2. Eight compounds were isolated from cortex phellodendri amurensis polystyrene resin, silica gel, by physical-chemical methods and spectral analysis, and five compounds were identified.They were berberine(1), palmatine(2), obaculactone (3),β-sitosterol(4), daucosterol(5).3. The quality control methods of cortex phellodendri amurensis were developed.(1) HPLC method was developed for berberine and palmatine, and the ranges of contents were established.(2) Colorimetry was used to determine the total alkaloids, and the limit range was established.(3) HPLC method was developed for obaculactone, and the range of content was established.4. HPLC-FPS analysis of cortex phellodendri amurensis was done with UV detector apparatus. Based on the results of methodology, the HPLC-FPS were established.5. By comparing the diversity between Phellodendron amurense Rupr. and P. chinensis Schneid. from different areas, the quality control and pharmaceutical activities were studied.6. At last, the preparation technology was established. These results can be served as basement for the further study of cortex phellodendri amurensis. They also afford reliable experimental basis for the application of HPLC in quality control of Traditional Chinese Medicine.