Effects Of Voluntary Exercise On Learning And Memory, Neurogenesis And Related Factors Of Infant Mice

Objective: Exercise can promote the function of learning and memory, but the specific mechanism still is not quite sure. Neurogenesis is to point to the generation of neurons, the process including neurons cell proliferation and migration, differentiation and live several and so on, each of the steps are likely to affect the new cells and the number of new neurons in the final. Hippocampal Neurogenesis maybe promote the ability of learning and memory. At present, a large number of experimental research shows that Sports training can promote Hippocampal Neurogenesis, and improve the ability of learning and memory. NCAM and SCF are important genes in the hippocampus, they can play an important role in Hippocampal Neurogenesis, so They can be used as landmark gene in Hippocampal Neurogenesis. In this article, Morris water maze and real-time quantitative PCR were used to test the effect of eight weeks independent movement on the infant mice in learning and memory ability and genes related with Hippocampal Neurogenesis mRNA expression level, exploring the function and meaning of neurogenesis in the process of exercise training improved the ability of learning and memory.Research methods:In this experiment,12screening mice were randomly divided into exercise group (R group, n=6) and control group(C group, n=6). The mice of R group run for voluntary exercise every day for eight weeks. At the eighth week, Morris water maze was used to all mice to test their learning and memory ability. All mice were killed off and the hippocampus were taken from their brains. Then we used RT-PCR to test the mRNA expression levels of hippocampus NCAM, SCF, IGF-1and VEGF of all mice.Experiment Results:(1) Through the Morris water maze testing, we found that the learning and memory ability of R group mice were better than C group mice.(2) After8weeks voluntary exercise, the mRNA expression levels of hippocampus NCAM of R group mice were significantly higher than those in C group mice(p<0.05).(3) After8weeks voluntary exercise, the mRNA expression levels of hippocampus SCF of R group mice were significantly higher than those in C group mice(p<0.05).(4) After8weeks voluntary exercise, the mRNA expression levels of hippocampus IGF-1of R group mice were significantly higher than those in C group mice(p<0.05).(5) After8weeks voluntary exercise, the mRNA expression levels of hippocampus VEGF of R group mice were significantly higher than those in C group mice(p<0.05).Conclusion:(1) Eight weeks voluntary exercise can improve the learning and memory ability of infant mice.(2) After8weeks voluntary exercise, the mRNA expression levels of hippocampus NCAM, SCF of R group mice were increased, we infer that Hippocampal Neurogenesis of the R group mice are more than the C group mice, indicating that eight weeks voluntary exercise can promote Hippocampal Neurogenesis of infant mice, then hint, the promoting function that eight weeks voluntary exercise can improve the learning and memory ability may be related to with Hippocampal Neurogenesis.(3) Eight weeks voluntary exercise can promote the mRNA expression levels of hippocampus IGF-1, indicating that eight weeks voluntary exercise can promote Hippocampal Neurogenesis of infant mice through improving the mRNA expression levels of hippocampus IGF-1.(4) Eight weeks voluntary exercise can promote the mRNA expression levels of hippocampus VEGF, indicating that eight weeks voluntary exercise can promote Hippocampal Neurogenesis of infant mice through improving the mRNA expression levels of hippocampus VEGF.