Biology Research Of The Aerobic Exercise And The Extracting Solution From AconitumszEchnyianum Gay Influence To The High Fat Diets Rats Vascular Endothelial Cell

Objective: Discuss the influence of aerobic exercise and the extracting solution from aconitum szechnyianum gay to the high fat diets rats vascular endothelial cell.Methods: Selects SD rats, weight 220±20g. random subdivided into seven groups: the rest control group (A group), the aerobic exercise group (B group), the high fat control group (C group), the high fat aerobic exercise group (D group), the high fat adds medicine group (E group), the rest adds medicine group (F group), and the high fat aerobic exercise adds medicine group (G group),each group of 10 rats, they eat and drink freely. A, B and F group was fed with common feed, other group was fed with high fat diet. B group starting exercise from the 1st week after the adaption to feed, D and G group starting exercise from the 5th week. The exercise intensity is the rats were trained 5d for each week, the initial velocity is 15m/min, when increases progressively speed 3m/min, time 5min, the movement speed is 20m/min, the time is 60min, maintains its exercise intensity, the training process continues 4 weeks; Starts A group, B group, C group and D group from the 5th week fills feeds the physiological saline, E group, F group and G group fills feed the extracting solution from aconitum szechnyianum gay. The rats freely drink and eat. Use microscope,electron microscope, immunohistochemistry and system of biological signals as well as automatic biochemistry analyzer to shape structure and the function change to measure and analysis vascular endothelial cell; the aerobic exercise and the extracting solution from aconitum szechnyianum gay as well as the aerobic exercise adds extracting solution from aconitum szechnyianum gay to the rats blood fats, artery blood stream dynamics and the vascular endothelial cell shape structure and the function influence. All data software package carry on processing using SPSS13.0 for the Windows. The statistical method includes: One-Way the ANOVA law, each of comparisons uses Independent-Samples T the Test law. The experimental result ((?)±SD) expressed by the mean value±standard difference, significant difference chooses P＜0.05 and P＜0.01.Results:1 The aerobic exercise and extracting solution from aconitum szechnyianum gay have a impact to the blood fats and weight, of high fat diets rats together with the different behavior performance.2 Through blood fats TC, TG, HDL and LDL index observation, hyperlipemia model establishment is effective; the aerobic exercise and the extracting solution from aconitum szechnyianum gay can reduce high fat diets rats blood LDL the density, advance the HDL density.3 Throughing the light microscope and the electron microscope observation, the high fat diets rats endothelial cell have the fat deposition, the partial endothelial cell appear the damage and have the plaque formation, the aerobic exercise and extracting solution from aconitum szechnyianum gay can improve the the fat deposition of rats endothelial cell, reduce the endothelial cell damage.4 Throughing light microscope observation, the aerobic exercise have the improvement function to the rats endothelial cell collagen; The extracting solution from aconitum szechnyianum gay is not obvious to the rats endothelial cell collagen.5 Blood plasma AngⅡand ET of rats obviously increase after the high fat diets, the aerobic exercise and the extracting solution from aconitum szechnyianum gay. may reduce AngⅡand the ET density.6 The high fat diets cause the rats arterial blood pressure to advance; the aerobic exercise can significantlly to reduce the high fat diets rats arterial blood pressure; After the medicine intervention, the high fat diets rats arterial blood pressure reduce; After the aerobic exercise and the medicine intervention synergism, the high fat diets rats arterial blood pressure have various degree. Showing that the aerobic exercise and the extracting solution from aconitum szechnyianum gay. affect arterial blood pressure of the high fat diets rats.7 ICAM-1, VCAM-1, CD31 and E-selectin all obviously advance in the high fat diets rats endothelial cell; the aerobic exercise can cause high fat diets rats endothelial cell ICAM-1, VCAM-1, CD31 and E-selectin expression reduce; After the extracting solution from aconitum szechnyianum gay interventions, ICAM-1, VCAM-1, CD31 and the E- selectin expression have various degree; the aerobic exercise and the extracting solution from aconitum szechnyianum gay synergism may cause high fat diets rats VEC VCAM-1, CD31, ICAM-1 and E- selectin expression significant change. Showing that the aerobic exercise and the extracting solution from aconitum szechnyianum gay to the vascular adhesion molecules the expression have significant influence.8 High fat diets rats VEC eNOS, iNOS and the nNOS expression can reduce the tendency; the aerobic exercise to be able to strengthen VEC eNOS, iNOS and the nNOS expression, also VEC eNOS, iNOS significant enhancement; After the extracting solution from aconitum szechnyianum gay interventions, high fat diets rats VEC eNOS expression significant enhancement, Showing that the extracting solution from aconitum szechnyianum gay have to the NOS production surrenders to the state the function; After the aerobic exercise and the extracting solution from aconitum szechnyianum gay coordination interventions, high fat diets rats VEC eNOS, iNOS and nNOS have various degree.9 High fat diets rats VEC AT-1, VEGF, NF-κB, TNF-a and TGF-βexpression significant advance; the aerobic exercise significant to reduce high fat diets rats VEC AT-1, VEGF, NF-κB, TNF-a and TGF-βexpression; the extracting solution from aconitum szechnyianum gay to high fat diets rats VEC AT-1, VEGF, NF-κB, TNF-a and TGF-βexpression have various degree to reduce the tendency; After the aerobic exercise and the extracting solution from aconitum szechnyianum gay dual stimulates, AT-1, VEGF, NF-κB, TNF-a and TGF-βexpression reduce.Conclusion: the aerobic exercise and the extracting solution from aconitum szechnyianum gay to the high fat diets rats blood fats, the VEC shape structure and the intercellular substance collagen, hemodynamics, AT-1, VEGF, NF-κB, TNF-a, NOS, ICAM-1, VCAM-1, CD31, AngⅡ, ET, TGF-β, E- selectin and so on have varying degree influence. The high fat diets may cause the rats VEC shape and the function change, the aerobic exercise and the extracting solution from aconitum szechnyianum gay may adjust the rats blood fats level, the change hemodynamics index, causes the shape structure and the function occurs remodeling. After causes this kind of change mechanism possibly is the high fat diets VEC active material AngⅡ, ET and NO secretion function change, the aerobic exercise and the extracting solution from aconitum szechnyianum gay may reduce AngⅡand ET secretion, strengthens NO the synthesis; AT-1, VEGF, NF-κB, TNF-a, ICAM-1, VCAM-1, CD31, TGF-βand E-selectin and so on vascular adhesion molecules and the cell active factor and the endothelial cell endocrine function close correlation, the aerobic exercise and the extracting solution from aconitum szechnyianum gay synergism can the varying degree adjustment and improves high fat diets rats VEC the shape structure and the function.