| Objective:Disorganized lipid metabolism causes direct consequence of cardiovascular disease in varied forms and the disease has become a hot spot of the world’s medical research. NO is the marker of vascular endothelial injury. Damaged vascular endothelial cell will cause disorders of endothelial function and the occurrence of a variety of cardiovascular diseases. Studies have shown that the increase of the combination of HSP90and c-Scr will promote the synthesis and secretion of NO, so as to maintain the normal operation of the function of vascular endothelial cell. Existing research experiments prove that exercises play an important role in the prevention and treatment of cardiovascular disease. Winter swimming becomes more and more popular, but no detailed reports show that whether the low temperature exercises affect the expression of HSP90and the activity of c-Scr. This study aims to observe the expression of vascular endothelial cells HSP90and the impact of c-Scr activity of high-fat diet rats caused by low-temperature exercises, investigate the impact of low-temperature exercises on fat decrease and cells endothelial function and provide definite theoretical basis for the prevention and treatment of cardiovascular disease.Methods:Choose247-week-old SPF male rats and feed with high-fat diet for six weeks. After six weeks, according to Obese Modeling Standard (the average weight of the rats fed with high-fat diet is20%higher than those fed with ordinary feed), choose23rats succeeding in modeling and randomly divide them into3groups which are Group A (high-fat control group),7; Group B (high-fat and low-temperature exercise group),8;and Group C (high-fat and room-temperature exercise group),8.Group B swims without load for50minutes at the temperature of (15±2)℃every day; Group C swims without load for50minutes at the temperature of (35±2)℃every day. The rats of both group will swim for6weeks (6times each week) and will be weighted every week during the experiment. After six weeks, the rats of each group were measured in serum NO, TC, TG, HDL and LDL indicators, as well as the expression of aorta vascular endothelial cells HSP90and the activity of c-Scr. Result:1. A group (high-fat control group), the body weight of rats was significantly increased (305.75g vs.364.57g); B group (high-fat low-temperature exercise group), a slight increase in body weight (317.00g vs.340.88g), but the growth rate is small. C group (high fat exercise group at room temperature), weight decreased slowly (308.38g VS293.14g).2. A group of rat liver (fat control group) was significantly higher than that in group B (high-fat low-temperature exercise group) and group C (high-fat at room temperature exercise group)(P<0.05, P<0.01); A group (high-fat control group) and group C (high-fat exercise group at room temperature) rat gastronomies’ weight was significantly lower than that in group B (high-fat low-temperature exercise group)(P0.05, P<0.01).3. B exercise group (high-fat low-temperature), C group (high fat exercise group at room temperature), TG level was significantly lower in group A (high-fat control group)(P<0.01); B group (high fat low-temperature exercise group) LDL levels compared with group A (high-fat control group) was significantly lower (P<0.05); HDL levels of the B group (high-fat low-temperature exercise group) and group C (high fat exercise group at room temperature) have increase in group B (high-fat low-temperature exercise group) was significantly higher than group A (high-fat control group) level (P<0.01).4. B exercise group (high-fat low-temperature) and group C (high-fat exercise group at room temperature) compared to the serum concentration of NO in group A (high-fat control group), have increased, but there was no significant difference.5. B group (high-fat low-temperature exercise group) and group C (high-fat exercise group at room temperature) the expression of HSP90amount than in group A (fat control group), group B (high-fat low-temperature exercise group) and group A (fat control group), was significantly increased (P<0.05) compared. Group B (high-fat low-temperature exercise group) rat aortic vascular endothelial cells HSP90expression and serum levels of NO Pearson correlation coefficient of0.950, P<0.01; C group (high fat exercise group at room temperature) rat aortic endothelial cells HSP90expression’ and serum levels of NO Pearson correlation coefficient of0.910, P <0.01. So that the aortic endothelial cells HSP90expression levels and serum levels of NO changes were positively correlated.6. B group (high-fat low-temperature exercise group) and group C (high-fat exercise group at room temperature), the expression of c-Src amount than in group A (fat control group), group B (high-fat low-temperature exercise group) the expression of c-Src with group A (high-fat control group), was significantly increased (P<0.01) compared. Group B rat aortic endothelial cells the expression of c-Src and serum levels of NO Pearson correlation coefficient0.912, P<0.01; C group (high fat exercise group at room temperature) rat aortic vascular endothelial cells c-Src expression The amount and the serum levels of NO Pearson correlation coefficient of0.914, P<0.01. So one could argue that the aortic endothelial cells the expression of c-Src and serum NO level was positively correlated.Conclusion:1. Room temperature swimming can significantly reduce the weight swimming exercise reduced fat, low temperature increase muscle, reduce body fat rate effect is more obvious.2. Movement can reduce serum TC, TG, LDL level, increase HDL levels, the more obvious effects of swimming in cold water movement.3. Movement can promote the formation of NO content, the temperature of its no significant impact.4. Movement can increase the expression level of the aortic endothelial cells of HSP90, a high correlation with the level of NO, and wherein the cryogenic significant effect. Swimming in low-temperature is more efficient than the normal temperature swimming to improve endothelial function.5. Movement can increase the amount of aortic endothelial cells in the expression of c-Src, and have a high correlation with the level of NO, and wherein the cryogenic significant effect. Swimming in low-temperature is more efficient than the normal temperature swimming to improve endothelial function. |
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