Effects Of Hypoxic Exercise On Inflanmmatory Factors TNF-α And IL-6 In Food-Induced Obese Rats

Objective: To establish a simple model of food-induced obesity induced by high-fat diet,to explore the relationship between obesity and serum inflammatory factors TNF-α,IL-6 and hypoxic exercise in serum inflammatory factors TNF-α and IL-6 in obese rats.The influence of 6 provides a theoretical basis for obesity patients to control body weight and regulate inflammation in vivo.Experimental methods: Eighty SPF male Sprague-Dawley rats were selected as experimental subjects.They were randomly divided into normal diet group(n=10)and high-fat diet group(n=70).All rats were free to eat during modeling.After 16 weeks of feeding,the body weight was greater than 10% of the average body weight of the control group and the Lee’s index was significantly larger than that of the control group.The obese rats were then randomly divided into an normoxia group(NQ,n=7),an normoxic group(NE,n=7),a hypoxic group(HQ,n=7),and a hypoxic group(HE).,n=7).The NE and HE groups underwent horizontal treadmill movement at 25m/min and 20m/min in normoxia and hypoxia,respectively,1 h/d,6d/week,continuous exercise for 5 weeks,and NQ and HQ groups did not exercise.The HQ group and the HE group were treated for 8 h/d in a hypoxic tent with an oxygen concentration of 12.8-13.6%,respectively.The HE group lived quietly for 7 h/d in a hypoxic environment,and exercised for 1 h/d.Both the NQ group and the NE group lived.In an atmospheric environment.The body weight,body length,and serum TNF-α and IL-6 levels were measured before intervention.After 5 weeks of exercise intervention,the body weight,body length,perirenal fat,epididymal fat and serum inflammatory factor TNF-α were measured.The levels of sputum and IL-6 were observed.The body weight and food intake of the rats were observed during the intervention and recorded.Experimental results: 1)Before intervention,the weight of the obese group was greater than 10% of the control group and there was a significant difference(P<0.01),and the Lee’s index was also significantly higher than the control group(P<0.05).Serum inflammatory factors TNF-α and IL-6 levels in the obese group were higher than those in the control group,and serum TNF-α levels were significantly different from the control group(P<0.05);serum inflammatory factors TNF-α,IL-6 levels and There was a positive correlation between body weight and Lee’s index in obese rats.Serum IL-6 was highly positively correlated with body weight and moderately positively correlated with Lee’s index.Serum TNF-α was moderately related to obese rats’ Lee’s index and body weight.Positive correlation(P<0.01).2)After 5 weeks of exercise intervention,the body weight,Lee’s index and fat coefficient of the exercise group were significantly lower than those of the quiet group(P<0.01).Among them,the hypoxia exercise group had the lowest body weight,Lee’s index and fat coefficient,and normoxic exercise.Secondly,the weight of the hypoxic exercise group was significantly lower than that of the normoxic exercise group(P<0.05).3)After 5 weeks of exercise intervention,the levels of serum inflammatory factors TNF-α and IL-6 in the exercise group were significantly lower than those in the quiet group.The serum IL-6 levels in the HE group and the NE group were significantly lower than those in the HQ group and the NQ group(P<0.01).Serum TNF-α was the lowest in the hypoxic exercise group,and there was a significant or significant difference compared with the NQ and HQ groups(P<0.01,P<0.05).Experimental results: 1)The serum TNF-α level of obese rats was significantly higher than that of the control group,and the body weight and Lee’s index of obese rats were positively correlated with serum TNF-αand IL-6 levels,suggesting that there was an inflammatory reaction in obese rats.2)The levels of serum inflammatory factors IL-6 and TNF-αin rats with chronic aerobic exercise were decreased in hypoxic environment.The mechanism may be related to weight loss and body fat and Lee’s index,suggesting that the hypoxic regimen of this study is more common.Oxygen lowers body weight and improves inflammation in obese rats.