Study Of Biotransformation Of Major Ginsenosides To Minor Ginsenosines By Microorganisms

Ginseng is a traditional Chinese medicinal herb. In recent years, many studies have shown that the minor ginsenosides(such as Rh2, Rg3, C-K, etc.) have strong physiological activity, and has been demonstrated to play a unique role in the anti-tumor. In this paper, several screened strains convert major ginsenosides to minor ginsenosides.In this study, we isolated 298 kinds of microorganisms from the soil and screened 176 (3-glucosidase producing strains. The 176 strains biotransformed the major ginsenosides Rbl, Rb2, Rc, Rd and Rg1 to minor ginsenosides. We screened out 19 strains which could convert ginsenoside Rbl to C-K; 17 strains which could convert ginsenoside Rb2 to C-Y; 4 strains which could convert ginsenoside Rc to Mc,1 strain which could convert ginsenoside Rc to Rh2,10 strains which could convert ginsenoside Rc to C-K; 1 strain which could convert ginsenoside Rd to Rh2,17 strains which could convert ginsenoside Rd to C-K; 3 strains which could convert ginsenoside Rgl to Fl and 2 strains which could convert ginsenoside Rgl to PPT. Among them, the strains GH9, GH21 and GH26 had the higher bioconversion rates.The strains GH9, GH21, and GH26 for the transformation of ginsenosides conditions were optimized. The optimum medium of GH9 is YB, the pH is 4.0-6.0, the optimum temperature is 40℃. The optimum medium of GH21 is H2O, the pH is 3.0-6.0, the optimum temperature is 40-60℃. The optimum medium of GH26 is YB, the pH is 4.0-8.0, the optimum temperature is 60℃.The ginsenosides Rb1 and Rd bioconversion rates by the strain GH9 were 86.1% and 52.6%, respectively. The ginsenosides Rbl, Rd and Rg1 bioconversion rates by the strain GH21 were 82.7%,63% and 97.8%, respectively. The ginsenosides Rb1 and Rd bioconversion rates by the strain GH26 were 76.6% and 40.5%, respectively.We had ITS sequencing of high performance strains, and had 16S rDNA sequencing. According to the sequencing results to construction phylogenetic trees, and combination with morphological to analyze strain GH9 as Penicillium, strain GH21 as Cladosporium and strain GH26 as Fungal. For analysing ginsenosides and their transformation products accurately, HPLC methods was discussed in this paper. We got a perfect HPLC method:CH3CN and H2O as the fluxion phase, C-18 column,203 nm as detection wave length and 1 mL/min as the flow rate.