Preparation And Quality Study Of Lutein Oil Microcapsule

Lutein is major caroteniod in nature.It is a kind of natural pigments with strong colouring power and antioxidation ability, meanwhile Lutein posseses many other significant physiological functions. There is ample epidemiological evidence that Lutein plays a positive role in the prevention macular caused by age,as well as cancer and cardiovascular diseases in human. Currently, it become a hot topic in functional food research. And it has been widely used in food and industries. However,its application in food industry is limited by the instability towards oxygen,light and temperature due to the eight-conjugated-double-bonds structure. On the other hand,the poor solubility is another obstacle, resulting in the actual production of Lutein in terms of application and storage has been greatly restricted.The microcapsules of Lutein can effectively solve these problems. In this paper,microeapsules of Lutein were made with spray-drying method in order to raise the stability of Lutein and make it more convenient in storage and applications. The preparation processing was studied, While the correlation quality research of the microcapsules were analyzed.Our purpose is to obtain microcapsules of high quality and high embedding rate of Lutein. It provides guidance to the industrial production of microcapsules.In the studying on spray drying processing technology of Lutein microcapsules, it were prepared by homogenization,emulsification and spray-drying using Lutein oil as the corematerial and sodium caseinate,whey powder in combination with sucrose as the wall material. By using the average encapsulation rate and shape of microcapsule as control, the single factorial tests were carried out.The effect of sucrose concentration,the ratio of core material to wall material wall and air inlet temperature on embedding rate were studied in detail and determine the optimal range:the wall material concentration is20%-60%,core-wall ratio is1:1-1:5, inlet air temperature is160℃-200℃, homogenizing pressure is50-70Mpa, the feed flow rate is8-12ml/min.On the basis of single factor experiment,response surface analysiswas used to optimize the process conditions including three independent variables sucrose concentration,the ratio of corematerial to wall material wall and air inlet temperature. The optimal conditions were obtained for the Lutein microencapsulation by spray drying method:Lutein Oil17.9%,29.7%sodium caseinate, whey protein powder29.7%,20.8%of sucrose, sucrose esters0.1%, Vcl.8%, solids content30%, inlet air temperature160℃, pressure homogenization60Mpa, homogenized three times, and feed rate5ml/min. And spray dried microcapsules with the feed temperature about50℃,The theoretical optimal embedding rate was96.2%,the embedding rate in practice by verifying was94.7%.The physical and chemical properties of Lutein microcapsules were also investigated using particle size analyzer and scanning electron microscope(SEM).The results showed microcapsules are yellow powder with uniform particles. Because of the low moisture content, it is suitable for long-term storage. Most of the resulting microcapsules particle surface are smooth and its external basic structure are nearly spherical by observing the product with SEM. Its angle of repose is43.7°, which has good flowability.In the control of raw materials, the stability of Lutein microcapsules including impact factor test, accelerated testing,long-term test and predictive validity were investigated. The stability of Lutein microcapsules are much higher than the raw material. According to Arrhenius empirical formula, we calculated the microencapsulated lutein valid for1.92years at25℃.The method for the delivery determination of Lutein microcapsules in the artificial gastric juice and artificial intestinal juice were established. Experiments results show that the microcapsules release faster in the artificial intestinal juice.Lutein microcapsules release meets the first order dynamic model in the artificial intestinal juice. Drug release equation is ln(1-Q)=-0.1143t-0.3066, the correlation index is r=0.9906. It release meets the zero order dynamic model in the artificial gastric juice. Drug release equation is Q=0.0129t-0.0318, the correlation index is r=0.9573.