Quinine And Salen High Performance Liquid Chromatographic Chiral Stationary Phases

The main work of this thesis was as follows:The significance and methods of chiral separation were introduced. The chiralstationary phases of high-performance liquid chromatography were mentioned. Thequinine derivative and Salen ligand were reviewed.Synthesized the quinine chiral stationary phases of high-performance liquidchromatography, the racemates of1,1’-bi-2-naphthol and fuloin were separated byusing methanol/0.5mol/L ammonium acetate (80:20) as mobile phase, respectively.We also modified quinine by p-methylphenyl chloride or3,5-dimethylbenzeneisocyanate for high-performance liquid chromatography chiral stationary phases, theenantioseparation of hydrobenzoin or omeprazole on the two chrial columns may beobtained.The N,N’-bis(2,5-dihydroxyl-salicylidene)-1,2-cyclohexanediamine and N,N’-bis(2,3,5-trihydroxyl-salicylidene)-1,2-cyclohexanediamine were prepared. In orderto study their chromatographic characteristic, two Salen derivatives were bonded tothe surface of amino propyl silica gel with diisocyanate. Some isomer sample suchas lodoaniline and bromaniline were separated on the columns.