Study On Novel Compound Botanical Sterilization Disinfectant

With the stepwise enhancement of ecological and environmental protectionconsciousness, people are raising higher and higher requirement about the quality ofdisinfectants. Therefore, on the premise of ensuring effective sterilization, it hasbecome a current research hot spot to reduce the use of chemical sterilizationdisinfectant and find new efficient environmental-protection disinfectants. The activeingredients compound of different antibacterial plants can expand the antibacterialspectrum and be not easy to make bacteria resistant. The compound of antibacterialplant active ingredients and a small amount of chemicals can not only use the efficientand readily availability of chemical sterilization, but also make use of theenvironmental protection, complexity, broad spectrum antimicrobial properties andnot easy to develop resistance of botanical active ingredients, thus so as to achievecomplementary and develop a new type of efficient and environmental protectioncompound disinfectant.On the basis of many reference literatures, active substances with a betterantibacterial ability, such as baicalin, honeysuckle, rhubarb, berberine and sophora,ect., were selected in this paper. With Escherichia coli (E. coli) and Staphylococcusaureus (S. aureus) as the tested bacteria, the minimum inhibitory concentration (MIC)determination test (agar dilution method) was used to determine the MIC of singlesubstance and joint MIC of two combined substances, then the fractional inhibitoryconcentration index (FIC) was calculated. According to the decision criteria of FIC,the joint antibacterial effect of different substances was obtained. The results show theantibacterial activity of the selected substances is in the order listed below:berberine> baicalin=honeysuckle> sophora> rhubarb. Moreover, by the compoundof two different substances, it has been gained that the joint of sophora, berberine,baicalin and honeysuckle shows the collaborative antibacterial effect on the two testedbacteria. Their joint MIC values are25mg/ml and18.4mg/ml, respectively, in whichmass ratio of sophora, berberine, baicalin and honeysuckle is about10:1:4:1and5:1:2:4, respectively.At the same time, by using trenching antibacterial test method, the dosage oftriclosan was determined, and we use it and the compound of sophora- berberine-baicalin-honeysuckle (mass ratio10:1:4:1) as the active ingredients ofcompound disinfectant. Moreover, through the stability test, antibacterial test andappearance observation, the effects of the dosage of solubilizer and change of pHvalue on the appearance and antibacterial activity of disinfectant were investigated.The test results are as follows:(1) The disinfectant used plant compound with bestcombination and2.5mg/ml of triclosan as active ingredients has a good antibacterialactivity;(2) Tween-80as solubilizer can satisfy the stability requirement, and at theaddition amount of3%, disinfectant can have a better appearance and higherantibacterial activity;(3) In the control of pH range, the change of pH has no obviousinfluence on the antibacterial activity of this compound disinfectant, and thecompound plant disinfectant with a better appearance and higher antibacterial abilitycan be obtained by adjusting the PH of the phosphate buffer (PBS) to8.0.Finally, the physical properties, antibacterial ability, water-reducible and stability ofthe compound plant disinfectant were evaluated in this paper. Results show that thecompound disinfectant is deep red, clear and odorless liquid. Its pH value is of7.64±0.02, the viscosity of (1.55±0.01) mPa·s, the average particle size of about310nm,and the effective ingredients with good dispersion. The compound disinfectant has agood antibacterial and bactericidal effect on E. coli and S. aureus, and it haswater-reducible and good stability, which can meet the requirements of antibacteriaand storage, etc.