High Performance Liquid Chromatography(HPLC) Method To Break Up Sex Drug Research

The chiral compounds was very common phenomenon in nature. With the development of science and technology, the understanding of enantiomer separation was in-depth in chemistry and the catalytic asymmetric synthesis technology, the separation and analysis of enantiomers was becoming important. Many chiral drugs may differ in their pharmacological activities due to the difference of stereochemistry. Therefore, rapid and sensitive methods for the resolution and quantification of the individual optically pure enantiomers are of great importance; chromatographic methods on both the analytical and preparative scale have become considerably interest in recent years. In this pape, The enantiomeric resolution of Cefadroxil, Mechanism acid and synephrine, pharmaceuticals being widely used in clinic were studied using chiral mobile phase additive(CMPA) by high performance liquid chromatography(HPLC). The effects of mobile phase, the kinds of CMPA and concentration, p H and flow rate have been investigated. The resolution mechanisms have also been discussed further. The resolution mechanisms have also been discussed further. The method of the separation of Cefadroxil, Mechanism acid and synephrine enantiomers were established by HPLC on a C18 column with hydroxypropyl-β-cyclodextrin(HP-β-CD) used as chiral mobile phase additive in the mobile phase. The results show that the separation of Cefalexin enantiomers was obtained when the mobile phase was composed of methanol:acetonitrile:aqueous phase=10:10:80(aqueous phase was composed of 16 mmol/L HP-β-CD, pH4.93), at the flow rate of 0.30 m L/min. At this moment, two pairs of enantiomers were separated. The method of resolution of Mechanism acid by HPLC with HP-β-CD as an additive in the mobile phase was that using the mobile phase consisted of CH3CN- H2O( 30:70), flow rate of 0.20 m L/min. The resolution of Mechanism acid was more than 1.30. Separationofsynephrine was the best with moble phase(methanol:acetonitrile:aqueous = 5:5:90in volum ratio, pH 7.10) contained 30 mmol/L HP-β-CD and flow rate of 0.80 mL/min. At this moment, one pair of enantiomer was separated entirely. The result showned that the method proposed was accurate, rapid with high resolutions.