Preparation Of Cationic Dietary Fiber And Its Adsorption On Bile Acid

Cationic polydextrose was prepared with a quaternary ammonium salt Q-188,3-chloro-2-hydroxypropyl-trimethyl ammonium chloride as cationic etherifying agent, polydextrose, fructooligosaccharides and polydextrose galacto-oligosaccharid-esin soluble dietary fiber as raw material, ethanol as solvent,under alkaline conditions. Degree of cationic substitution was determined with semi-micro Kjeldahl method, and their structures were characterized by FT-IR and13CNMR. Under the support of the known literature, extracted from fresh pig in bile acid and titration of the bile acid content were measured to calculate, plus the base of the bile acid anion. Finally, let cation dietary fiber and bile acid anion adsorption reaction, adsorption rate obtained under different conditions, the comparative study of the adsorption properties of bile acids. The main contents and results as follows:1.Quaternary Q-188at a temperature of40℃, pH9to10, anhydrous ethanol under etherification reaction solvent to produce2,3-propylene oxide chloride (Q-151).2.Cationic Dietary fiber was preparationed under basic conditions by Q-151and Dietary fiber, and by varying the amount of cationic etherifying agent, reaction temperature, reaction time, pH and other conditions to optimize the process for the synthesis of cationic dietary fiber, finalized the optimal reaction conditions: polydextrose and Q-188mass ratio of40:9, the reaction temperature is60℃,reaction time3h, pH of9or10, as measured under optimal conditions cationic degree of substitution of0.1333; low quality Q-188and polyfructose ratio20:15, the reaction temperature was70℃, reaction time2.5h, pH9to10, in this condition the degree of cationic substitution measured as0.1310; with Q-galacto188mass ratio of20:10, the reaction temperature is70℃, reaction time3h, pH9to10, the cationic degree of substitution of0.1404. And the target product was analysised by FT-IR and13CNMR and confirmed the synthesis of the compound is a cationic fiber.3.In support of the known documents extracted from the bile of swine bile acid crude product, the saponification process for the filter Saponificationâ†'acidificationâ†'washingâ†'bleachingâ†'acidified sulfate, and the content was determined by titration of bile acids in bile acid crude product, plus base of the bile acid anion.4.At a pH of7.8and a temperature of37～38℃constant conditions, so that the cationic and anionic dietary fiber bile acid adsorption reaction, and the absorption time by changing the amount of dietary fiber in an amount of cationic bile acid adsorption reaction optimum adsorption conditions were optimized, identified as: Cationic polydextrose5.0g, bile acid0.5g, adsorption3hours, maximum adsorption rate was61%; cation oligofructose1.0g, bile acid0.5g,2.5adsorption hours, maximum adsorption was69%; cation galactooligosaccharide2.5g, bile acid0.5g, adsorption three hours, the maximum adsorption rate of73%.5.Parallel under conditions to allow the dietary fiber before the modification with a bile acid anion adsorption reaction, the rate of absorption at the maximum absorption time changing conditions of polydextrose24%,33%fructo-oligosaccharid es, galacto-oligosaccharides35%.6.By experimental results, no matter what kind of dietary fiber, compared to the previous modification,after the adoption of cationic modified, the adsorption rate of bile acids have a very significant improvement,more than30%.