Analysis Of Polysaccharides From 20 Species Of Marine Shellfishes

Polysaccharides are important nutriceutials in shellfishes, and in the present study polysaccharides from 20 edible marine shellfishes of Bivalvia, Lamellibranchia, and Gastropod were studied. In the present study, polysaccharides from 20 edible marine shellfishes were identified and quantified by using existing methods and setting a new method for polysaccharides analysis. The results do not only give reference data for nutrition assessment of these edible shellfishes, but also provide the scientific basis for the explorment of shellfish polysaccharides.The Phenol-sulfuric acid method and 1,9-dimethyl-methylene blue method are used to determine the content of neutral sugars and sulfated polysaccharides in 20 edible shellfishes.A methodology of analyzing their disaccharides produced from the acid hydrolysis using HPLC-MSn upon 1-phenyl-3-methyl-5-pyrazolone(PMP) derivatization was set to identify the uronic acid-containing polysaccharides(UACPs). Specific process is as follows: first of all,based on the optimization of experimental conditions by the single factor experiment and orthogonal test design. Subsequently, these conditions were applied to investigate the distribution of UACPs in 20 shellfishes. PMP-disaccharides derived from UACPs in mollusks were identified by comparing the retention time and mass spectra with those of the reference PMP-disaccharides from hyaluronic acid(HA), chondroitin sulfate(CS), heparin(HP), and AGSP withâ†'4)-GlcA(1â†'2)-Man(1â†'repeating units. As a result we found the significant differences in the content of neutral sugars and sulfated polysaccharides in a variety of shellfishes. The neutral sugars content of Gomphina aequilatera and Rapana venosa is higher than other sheiifishes. Ruditapes phliippinarum Mytilus coruscus Gould and Buccinum pemphigum are high content of sulfated polysaccharides.Besides, the combination of 1.3 M TFA at 105°C for 3h is found to be the optimum. The analysis reveals the prevalence of CS in the shellfishes as well as the HP, and existence of three non-GAG UACPs in 7 mollusks.