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Technology Research Of Lead Contaminated Soil Phytoremediation Technology Of Phyllostachys Praecox

Posted on:2015-03-22Degree:MasterType:Thesis
Country:ChinaCandidate:J Y WangFull Text:PDF
GTID:2271330470477340Subject:Agricultural resource utilization
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Because of the increasingly intensified lead pollution, phytoremediation of soil lead(Pb) pollution is imperative.The study focused on the effects of Pb contaminated soil phytoremediation technology of Phyllostachys praecox. Phyllostachys praecox were found in lead-zinc mine, and be able to maintain good growth momentum of tree species, have very important research significance.Through this research, it was hoped that the results of Phyllostachys praecox’s phytoremediation ability could provide a new perspective for heavy metal contaminated soil phytoremediation technology development in the future.Through hydroponics and soil culture experiments, the absorption, accumulation and transport capacity under lead stress were studied, and the photosynthesis levels and ultrastructural characterization of Phyllostachys praecox were analyzed..The main results are described as follows:(1) Phyllostachys praecox can absorb a high concentration of Pb, and the Enrichment of underground part quantity increases with the increase of Pb concentration. In a hydroponics experiments, Pb concentrations reached to the maximum of 6706, 3710, 26388 mg·kg-1 in leaves, stems and roots at the Pb content of 100μmol·L-1, in soil culture experiments as well as 63, 101, 595 mg·kg-1 in leaves, stems and roots at the Pb content of 1200 mg·kg-1 in soil culture experiments.(2) High levels of Pb could damage the ultrastructure levels of Phyllostachys praecox in a certain degree, and Have inhibition on photosynthesis, but the growth of Plant is normal. This phenomenon Show that Phyllostachys praecox can patience with a high levels of Pb.Through observing Phyllostachys praecox chlorophyll fluorescence, it was found that the values of [Y(Ⅱ)] and ETR decreased, which indicated that Pb stress inhibits photosynthetic product formation and electron transfer capacity of Phyllostachys praecox, while the impact was not significant on plant biomass. Through the observation of the ultrastructure levels of Phyllostachys praecox by TEM under Pb stress, it could be found that the damage is not obvious on plant tissue under low levels of Pb. However, under high levels of Pb the part of the cell wall deformed, and the chloroplast membrane disappeared or ruptured partially, and the lamellar structure blurred.(3) The accumulation increases with the increase of Pb concentration in the root department of Phyllostachys praecox, but the Pb accumulation increase is not obvious on the ground. Transfer coefficient was reduced by 34%. This phenomenon can prove that the heavy metal-Pb can restrain the process of Pb from root department transfer to the ground department with the increase of concentration of Pb.Although Phyllostachys praecox is not hyperaccumulator, but it have several characteristics, such as high biomass, strong reproductive capacity, high economic value and great market prospects and so on. In phytoremediation market Phyllostachys praecox have huge potential of heavy metal contaminated soil.
Keywords/Search Tags:Phyllostachys praecox, Heavy metal, Lead(Pb), Phytoremediation, Accumulation, Transmission electron microscopy
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