Study On Extraction Technology, Antioxidant And Structural Analysis Of Polysaccharide From Cynomorium Chinense In Ningxia

Cynomorium belongs to all the grass family one genus, there are usually many alias show iron etc. At the same time it is parasitic seed plant. The medicinal part of Cynomorium songaricum Rupr as its to inflorescence dry stems, it is more common in the distribution Chinese climate in arid desert area, such as Ningxia, Inner Mongolia and other places. Cynomorium songaricum Rupr. Also called the "desert ginseng", "elixir", "precious", with the help of kidney yang, solid essence and blood, Runchang Tongbian, anti-aging and other a series of medicine efficacy. Review of the literature has been reported rarely chemical composition on Cynomorium songaricum Rupr in recent years.This experiment selected Ningxia areas of Cynomorium songaricum Rupr by water extraction and alcohol precipitation method to obtain the crude polysaccharide, and the crude polysaccharide was separated and differentiation, structure identification. In addition, this experiment has researched of Cynomorium songaricum polysaccharide in vitro antioxidant activity. The conclution are as follows:(1) Using the traditional method of water extraction and alcohol precipitation of Cynomorium songaricum polysaccharide extraction, and based on the single factor experiment, the extraction technology was optimized by response surface method, the following two multivariate regression model equation: Y=21.81+0.36×X1+0.41×X2-0.30×X3+0.31X1×X2-0.26×X1×X3+0.44×X2×X3-0.82×X12-0.35×X22-0.84X32 The optimized process parameters were:the extraction temperature of 90 ℃, extraction time 2.5h, ratio of material to liquid 1:25 (w/v=g/mL), under the condition of actual test of Cynomorium songaricum polysaccharide extraction rate was 22.07%.(2) The results of in vitro antioxidant of Cynomorium songaricum polysaccharide showed that, the total reducing power and has the 3 kinds of free radicals (DPPH, O2-, OH) showed a scavenging effect. In addition, the test can be obtained the relevant from Cynomorium songaricum polysaccharide concentration on the size and their antioxidant activities.(3) Through (DEAE-52) cellulose chromatography separation, get all the different fractions (SephadexG-100), after Sephadex gel chromatography separation and purification, progress, get Cynomorium polysaccharide pure component of SCP-I, SCP-Ⅱ, SCP-Ⅲ and Ⅳ SCP-, high performance liquid chromatography (HPLC) validation for homogeneous polysaccharide component.(4) By high performance liquid chromatography (HPLC) analysis of molecular and purified polysaccharide component of SCP-Ⅰ, SCP-Ⅱ,SCP-Ⅲ, SCP-Ⅳ of molecular weights of 2.89 × 104Da,1.95 × 104Da,2.28 × 104Da,2.41 × 104Da.(5) Using the Fu Liye transform infrared spectroscopy (IR), to determine the SCP-of beta pyranose glycosidic bond configuration; SCP-Ⅱ, SCP-Ⅲ, SCP-Ⅳ alpha glucoside bond configuration.(6) Application of environmental scanning electron microscope (SEM) scanning electron microscope observation of the fourth homogeneous polysaccharide, respectively, in ×1000, ×2500 magnification of the internal structure and get the fourth homogeneous polysaccharide contained lamellar, spherical and granular component.