Study On Supercritical Fluid Extraction Of Alkaloids From Fritillaria Thunbergii Miq

In this paper, Fritillaria thunbergii flower and bulb were choiced as experiemental material.The toal alkaloids and monomer compositions were extracted by supercritical CO2 fluid. The total alkaloids content was determined by UV and the contents of peimisine, peimine and peiminine were determined by UPLC-ELSD. The working curves of total alkaloids, peimisine, peimine and peiminine were determined. The precision, stability and the rate of recovery of each method have good feasibility and it means all the method are available and effective.The effects of extraction time, extraction temperature, extraction pressure and ethanol concentration were explored. Second order quadratice quations for supereritical fluid extraction of alkaloids were built and combined with the practice of the actual operation, by using the new method discussed above. (1)The optimum extracting condition of total alkaloids, peimine and peiminine from Fritillaria thunbergii flower was as follows:extraction time 2.9h, temperature 61.3℃, pressure 30.6MPa, ethanol concentration 90.3% with yields of 2.94mg/g for total alkaloids,0.69mg/g for peimine and 0.0714mg/g for peiminine; (2) The optimum extracting condition of total alkaloids, peimisine, peimine and peiminine from Fritillaria thunbergii bulb was as follows:extraction time 2.98h, temperature 60.44 ℃, pressure 26.48MPa, ethanol concentration 89.31% with yields of 3.81mg/g for total alkaloids,0.488mg/g for peimisine, 1.25mg/g for peimine and 1.30mg/g for peiminine. At last, the experimental data were in good agreement with the predicted values. Thus, the models can reflect the extraction processes.In addition, the difference of alkaloids between flower and bulb was compared.