Analysis Of Chemical Constituents Of Fritillaria From Different Producing Areas And Application Of Fritillaria Chinensis In Health Food

Fritillaria is derived from a variety of dried bulbs of the genus Liliaceae,and has long been used as one of the most important antitussive,expectorant and antihypertensive drugs in Chinese medicine.It has a wide range of original plant sources and complex species.Inter-variation and significant pharmacodynamic differences.The Chinese Pharmacopoeia(2015 edition)contains 11 species of Fritillaria,including 6 species of Fritillaria,2 species of Fritillaria,Fritillaria,Fritillaria and Fritillaria.In this paper,a total of 7 samples from different origins of Fritillaria were selected and included,and the differences in active substances of Fritillaria from different producing areas were explored and quantitatively analyzed.It provides a valuable reference for the origin and variety of Fritillaria,which is suitable for the development of Fritillaria alkaloid products.The detection of the content of cilostatin in the dried bulbs of the four different producing areas by HPLC showed that the content of cilostatin in the No.1 medicinal material of Anhui Province was the highest,which was 0.084%,which was consistent with the reference results.It is generally considered that the Sichuan Fritillaria(No.3 medicinal material)with the highest content of cilostane is the second,and the content of cilostatin is 0.0570%.It is generally believed that the shellfish(Zhejiang No.7)of Zhejiang origin and the Fritillaria(No.6 medicinal material)from Hubei origin are tested to prove that the content is not much different from that of Sichuan Fritillaria(No.3 medicinal material).In the traditional Chinese medicine prescription,the content of cilantroin in the Xinjiang origin(the second medicinal material)and the Jilin origin(the medicinal material)in the Jilin origin are the lowest.As the cultivated product of Sichuan origin,the No.4 medicinal material has a content of cilostamine of 0.0526%,which is close to that of the medicinal material of the same origin in Sichuan.The above results indicate that the content of cilostane in Fritillaria has a certain correlation with its origin.The chemical constituents of the ethanol extracts of Fritillaria from different habitats were analyzed by GC-MS,and some chemical compositions were identified.Analysis shows that the dried bulbs of Fritillaria generally contain linoleic acid,linolenic acid,palmitic acid,octadecanoic acid and the like.Among them,the highest content of linoleic acid is the No.5medicinal material of Jilin Province,the relative content is 31.37%;the second is the No.3medicinal material of Sichuan,the relative content is 25.78%.The highest content of linolenic acid is the No.3 and No.4 medicinal materials in Sichuan,with relative contents of 9.19%and 9.09%,respectively.No linolenic acid was detected in the No.5 medicinal material of Jilin Province and the No.7 medicinal material of Zhejiang Province.None of the four components in the No.6 medicinal material in Hubei Province was detected,but it does notmean that these components do not exist in the medicinal materials.It can be known from the data in the Appendix table that they may exist in the form of lipids or organic acid salts.The analysis of the volatile components of Fritillaria provides an experimental basis for the development of health foods.The extraction process of Fritillaria acuminata was optimized.The extraction rate and method optimization were carried out for the heating reflux extraction method and the supercritical CO2 extraction method,respectively.The effects of various factors on the experimental results were the extraction temperature > extraction time > ethanol volume fraction > material-liquid ratio,and the extraction temperature had a significant effect on the experimental results.After optimization,the optimum conditions are extraction temperature70 ° C,extraction time 40 min,ratio of material to liquid 1:7,ethanol volume fraction of 75%.The optimal extraction rate was determined to be 0.0521%(calculated as the content of cilantamine).The effects of various factors on the experimental results of supercritical CO2 extraction method were extraction pressure>extraction temperature>extraction time.The extraction pressure and extraction temperature had significant effects on the experimental results.After optimization,the optimum conditions are extraction pressure 35 MPa,extraction temperature 45 ° C,and extraction time 40 min.The optimum extraction rate was determined to be 0.0503%(calculated as the content of cilantamine).The experimental results show that the heated reflux extraction method generally has a higher extraction rate.After the supercritical CO2 extraction method is optimized,the extraction rate is still low.The reason may be that the amount of raw materials added to the experiment is small,and the supercritical equipment pipeline is long,resulting in loss.The paper developed a kind of Chuanbeimu Runfei Cough Tablets,which provided ideas for the application of Chuanbei in health food.The experiment determined the ratio of raw materials and auxiliary materials,namely,mixed extract,soluble starch,magnesium stearate and talc powder,the best ratio was 45:30:2:23.The addition of 1.0% aspartame and 2.0%citric acid is the most popular.It was confirmed by thin-layer chromatography that all the Chinese herbal medicines in the tablets were detected,which proved that the experimental method was correct.The average tablet weight of Chuanbeimu Runfei Cough Tablets was1.10885 g,and the difference in tablet weight and disintegration time limit met the relevant standards.The content of the cilostane in the tablet was 0.18807 mg/g.