Study On Extraction And Separation Of Polysaccharide From Pomelo Peel And Moisture Character

The isolation, purification and molecules weight distribution of polysaccharides from pomelo peel, as well as their moisture effects and the safe assessment for tobacco were investigated in this study. The main contents and results are as follows:Aiming at optimization of the extraction of polysaccharides from pomelo peel, response surface methodology was adopted. The results showed that the optimum conditions of grape fruit polysaccharides extraction were as follows: extraction time of 2.2h. enzyme dosage 1.5%,enzymolysis time 120 min, solid to liquid ratio of 1:25, enzymolysis temperature 50 ℃.Under these conditions the extraction yield could be up to 10.39%.The purification and concentration of polysaccharides from pomelo peel were carried out via different organic solvent apartment. It was found that the lower concentration of ethanol and the higher the molecular weight of the precipitated polysaccharide. A peak of coarse polysaccharide(1) had different weight distribution for the heavy molecular weight Mw to155000 da, coarse polysaccharide peak b(1) the heavy molecular weight Mw to 137000 da,cpeak of coarse polysaccharide(1) the heavy molecular weight Mw to 39700 da.The moisture properties of polysaccharide from Pomelo peel on tobacco show that there is a certain relationship with polysaccharide concentration. The water phase in tobacco is measured by Nuclear magnetic for the absorption water on the surface and free water in tobacco tissue. Hygroscopic water are significantly reduced when four of tobacco samples were placed for 3 h, and reach to the basic balance for 6 h. And the free water of four tobacco tissue had different change trend.Different pomelo peel polysaccharide had no obvious effect on cigarette physical indexes,the routine chemical components, cigarette smoke components, also did not increase the harmful substances in the mainstream smoke.Safety evaluation showed that the content of heavy metals of pomelo peel extract was in the range of security, and there was not animal acute toxicity observed under the maximum oral dose. Which had not mutagenic effects in subjects doses without bacteria, respectively, in the largest oral doses(35.45 g/kg) induced bone marrow micronucleus rate has no obvious difference compared with blank control group.