Study On Fourier Transform Infrared Spectroscopy For The Determination Of Vitamin C Active Pharmaceutical Ingredient And Preparation

Vitamin C is an essential nutrient, and the determination of its content is an important index of quality control in food and pharmaceutical industries. In this paper, a new method for direct, rapid, pollution-free determination of vitamin C active pharmaceutical ingredients(APIs) and preparations was established by Fourier transform infrared spectroscopy(FTIR), and satisfactory results were achieved. The dissertation introduced vitamin C, its physiological and pharmacological functions, production process and determination methods, and summarized the application status of FTIR technique.Diffuse reflectance Fourier transform infrared spectroscopy(DRIFTS) was used for semiquantitative analysis vitamin C APIs and quantitative analysis vitamin C preparations. Whether vitamin C APIs were up to standard was rapidly tested by DRIFTS. Standard substances for determination of vitamin C solid and liquid preparations were vitamin C and sodium ascorbate, and potassium bromide(KBr) was used as the background in the determination of vitamin C preparations. The vibrational peak of C=O stretching at 1755cm-1 for vitamin C, 1702cm-1 for sodium ascorbate were selected for quantitative analysis vitamin C solid and liquid preparations. The absorbance were measured by peak area in regions of 1728~1782cm-1 and 1685~1736cm-1. With the optimized experimental condition, the absorbance and the concentration of vitamin C and sodium ascorbate showed good linear relation in the ranges of 0.2%~1.0% and 0.2%~1.6% with the regression equation Y=1.732X+1.291 and Y=1.912X+1.617(correlation coefficient, r=0.999, 0.997). Limits of detection were 0.2mg/g and 0.09g/m L, respecvtively. The recoveries were 98.0%~102.2%。Fourier transform infrared(FTIR) spectroscopy(KBr pellet) was used for semiquantitative analysis vitamin C APIs and quantitative analysis vitamin C preparations. Whether vitamin C APIs were up to standard was rapidly tested by FTIR spectroscopy(KBr pellet). Standard substances for determination of vitamin C solid and liquid preparations were vitamin C and sodium ascorbate in the determination of vitamin C preparations. The vibrational peak of C=O stretching at 1754cm-1 for vitamin C, 1702cm-1 for sodium ascorbate were selected for quantitative analysis of vitamin C solid and liquid preparations. The absorbance were measured by peak area in region of 1730~1782cm-1 and 1685~1735cm-1. With the optimized experimental condition, the absorbance and the concentration of vitamin C and sodium ascorbate showed good linear relation in the ranges of 0.2%~1.0% and 0.2%~1.6% with the regression equation Y=11.282X+0.646 and Y=20.087X+0.368(correlation coefficient, r=0.999, 0.997). Limits of detection were 0.03mg/g and 1.0×10-5g/m L, respecvtively. The recoveries were 98.3%~102.7%。The sequential injection analysis coupled with Fourier transform infrared spectroscopy(SIA-FTIR) was used for semiquantitative analysis vitamin C APIs and quantitative analysis vitamin C preparations. Whether vitamin C APIs were up to standard was rapidly tested by SIA-FTIR technique. Standard substances for determination of vitamin C solid and liquid preparations were vitamin C and sodium ascorbate, and Zn Se window filled with water was used as the background in the determination of vitamin C preparations. The vibrational peak of C=O stretching at 1759cm-1 for vitamin C, 1720cm-1 for sodium ascorbate were selected for quantitative analysis of vitamin C solid and liquid preparations. The recoveries were 98.2%~101.0%.The FTIR technique, for determination of vitamin C active pharmaceutical ingredients and preparations, is characterized by simple and rapid operation, accuracy of results, low consumption of reagents and samples, and no-pollution. It’s an environment-friendly analytical method.