| This paper established a rapid, which was easy to operate that can be used to measure the blueberry juice of 11 kinds of phenolic acids concentration in High Performance Liquid Chromatography; Using the established HPLC analysis testing blueberry fruit and the prod-ucts of blueberry juice; Using high performance liquid mass spectrometry technology to detect the type of anthocyanins, the composition and the concentration of the anthocyanins, of which northland blueberry, lonicera caerulea, waxberry, mulberry, blackcurrant, linberry, black chokeberry; study of the characteristics of each type of fruit anthocyanins; and carries on the preliminary adulteration of blueberry juice products. The main conclusions were as follows:1. This study selected and optimized the conditions of liquid chromatograph. using XAqua C18 (250x4.6mm,4βm), formic acid-acetonitrile-water solution as mobile phas-e, gradient elution and flow rate of 1.OmL/min, column temperature was 30℃. Establi-shed a rapidly and easily high performance liquid chromatography (HPLC) analysis, by which can be used to measure the concentration of 11 kinds of phenolic aicds of blueberryjuice.2. Using the established method of HPLC to test the homemade northland blueberries juice of different concentrations in lab, as a result,10%,30%,50%,70%,80%,100%con-centration of northland blueberry juice were all detected 11 kinds of phenolic acids. They are gallic acid, protocatechuic acid, gentian acid, P-hydroxy benzoic acid, vanillic acid, svringic acid, chlorogenic acid, caffeic acid, coumaric acid, ferulic acid, sinapic acid.3. In different concentrations of northland blueberry juice samples, the relative ratio of phenolic acids of different concentrations of blueberry juice was different. With the concen-tration of blueberry juice increased, the concentration of 11 kinds of monophenolic acid did not increase. But the concentration of phydroxy benzoic phenolic acids and hydroxy benzoic phenolic acids increased. And the concentration of hydroxy benzoic phenolic acids was al-ways greater than P-hydroxy benzoic phenolic acids, the proportion of which found no ob-vious change.4. In northland blueberry juice (100%), concentration of gallic acid was 0.19mg/mL, concentration of protocatechuic acid was 0.14mg/mL, concentration of gentian acid was 10.85mg/mL, concentration of P-hydroxy benzoic acid was 0.055mg/mL, concentration of vanillic acid was 11.93mg/mL, concentration of svringic acid was 0.12mg/mL, concentration of chlorogenic acid was 33.98mg/mL, concentration of caffeic acid was 1.29×10-4 mg/mL, concentration of coumaric acid was 5.05mg/mL, concentration of ferulic acid was 0.53mg/mL, concentration of sinapic acid was 0.61mg/mL. In different concentrations of northland blueberry juice, the concentration of chlorogenic acids was the highest, which the percentage of total phenolic acid concentration was 55%. Followed by gentisic and vanillic acids, which accounted for 17%, and 18%respectively.3 kinds of phenolic acids accounted for 91%of the concentration of total phenolic acids. Chlorogenic acids, gentisic and vanillic aicds can be used as the mainly phenolic acids of northland of blueberry juice.5.9 kinds of blueberry juice samples, which were testing the contents of phenolic acids by HPLC. It found that the kinds of phenolic acids of blueberry fruit were different from the products of blueberry juice. And the concentration of phenolic acids of the products of blu-eberry juice significantly lower than the concentration of phenolic acids in blueberry fruit. In 9 samples of blueberry juice, only the sample 2 (US) content of juice 100%, sample 4 (Great-er Khingan Mountains in heilongjiang province) content of juice 95%, sample 8 (Qingdao) content of juice 100%, which detected 11 kinds of phenolic acids. Remaining samples were lack of 1-3 kinds of phenolic acids, all of which were lack of chlorogenic acid.6. Northland blueberry was found to contain 17 anthocyanins by HPLC/MS. The 17 of them were tentatively identified as Delphinidin-3-galactoside,Delphinidin-3-glucos-ide, Cyanidin-3-galactoside, Delphinidin-3-arabinoside. Cyanidin-3-glucoside, Petunidin-3-gal-actoside, Cyanidin-3-arabinoside and Petunidin-3-glucoside, Petunidin-3-galactoside, Petunidin-3-arabinoside, Petunidin-3-glucoside and Malvidin-3-galactoside, Malvidin-3-gl ucoside, Malvidin-3-arabinoside, Peonidin-3-Acetylgalactoside, Malvidin-3-xyloside, Peon idin-3-Acetylgl-ucoside, Malvidin-3-Acetylgalactoside, Malvidin-3-Acetylglucoside. The mainly anthocyanins of Northland blueberry juice were Malvidin-3-galactoside and Mal-vidin-3-glucoside.7. Detecting the anthocyanins of lonicer caerulea. black currant, waxberry, mulberr-y, linberry, black chokeberry by HPLC-MS. Lonicer caerulea was foud to mainly cont-ain 3 kinds of anthocyanins. The 3 of them were tentatively as Cyanidin-3-galactosid-e, Cyanidin-hexose and Malvidin-hexose, Cyanidin-hexose was the characteristics of a-nthocyanins of lonicer caerulea. Black currant was found to mainly contain 2 kinds o-f anthocyanins. The 2 of them were Delphinidin-hexose and Cyanidin-hexose. Both D-elphinidin-hexose and Cyanidin-hexose were the characterristics of anthocyanins of bla-ck currant. Waxberry was found to mainly contain 2 kinds of anthocyanins. The 2 of them were Delphinidin-3-glucoside and Cyanidin-hexose. Cyanidin-hexose was the ch-aracteristics of anthocyanins of waxberry. Mulberry was found to mainly contain 2 kin-ds of anthocyanins. The 2 of them were Cyanidin-3-glucoside and Cyanidin-hexose.C y-anidin-hexose was the characteristics of anthocyanins of mulberry. Linberry was fou- nd to mainly contain 2 kinds of anthocyanins.The 2 of them were Cyanidin-3-glucosi-de and Cyanidin-hexose. Cyanidin-hexose was the characteristics of anthocyanins of li-nberry. Black chokeberry was found to mainly contain 2 kinds of anthocyanins. The 2 of them were Cyanidin-hexose and Cyanidin-arabinoside. Cyanidin-hexose was the c-haracteristics of anthocyanins of black chokeberry.8. Detecting the types and content of anthocyanins of 9 different producing area of blu-berry juice samples by HPLC-MS. As a result, the sample 1 (Greater Khingan Mountains in heilongjiang province) content of juice was more than 60%, Sample 3 (Bulgaria) content of juice was 10%, sample 4 (Greater Khingan Mountains in heilongjiang province) content of juice was more than 95%, sample 5 (Greater Khingan Mountains in heilongjiang province) content of juice was more than 80%, sample 6 (Dandong in liaoning province) content of juice was 100%, sample8 (Qingdao in Shandong province) content of juice was 100%, Sam-ple 9 (Changbai Moutain) content of juice was more than 90%, which the kinds and propor-tions of anthocyanins were accord with blueberry fruit. It determined that those samples were not adulterated. As the different of planting conditions, picking time and processing technol-ogy, sample2 (US) content of juice was 100%and sample7 (Canada) content of juice was more than 100%, which belonging to foreign varieties, that can not be determined wether added to blueberries. |
