Research On Decolorization Of Reactive Dyes By Anti-nutritional-Repression White Rot Fungi Under Non-Sterilized Condition

In recent years.with the rapid development of textile industry,the varieties and quantity of dyes increase continuously and the structures are complicated.Of all the dyes, the reactive dye has become a kind of the most important dyes because of its complete color spectrum,bright-coloured colour and lustre, strong adaptability,low price and excellent color fastness.Besides,it is easy to use and accords with environmental requirement.How to search the method of degrading reactive dye waste water has always been a focus vast numbers of researchers care.White rot fungi are cared in waste water treatment by researchers because of their unique enzyme activity degradation mechanism.How to degrade reactive dye waste water efficiently under non-sterilized condition is the top priority.This thesis takes Phanerochaete Chrysosporium 553,the type strain of white rot fangi,CFCC-5336 of Coriolus versicolor and the six strains mutated from CFCC-5336 of Coriolus versicolor as the material.The excellent strain which has the best degradation effect on reactive dye waste water was selected under non-sterilized condition.Then we carried out the research on its morphological character and enzyme-production characteristic and determined the best technological parameters and effect factors of its enzyme production.Besides,we made a further study of its continuous intermittent culture and its treatment efficiency on compounded dyes.Through research,we reached the following results:(1) Select six kinds of white rot fungi from the laboratory(Pc555,5324,5327,5305, Coriolus ersicolor 5336, CFCC-4) and use them to degrade four kinds of simplex reactive dyes(reactive brilliant red X-3B, λmax=513nm; reactive brilliant red KD-8B, λmax=545nm reactive brilliant blue KN-R, λmax=568nm; reactive brilliant yellow K-6G, λmax=410nm).Contrasting the decolorization degradation effect,we found that these six kinds of white rot fungi thalli all have certain degradation effect.But contrasting these six kinds of thalli, CFCC-4 of Coriolus versicolor is the best strain to degrade these four kinds of dyes. The decolorization rate of reactive brilliant red X-3B、reactive brilliant red KD-8B、reactive brilliant blue KN-R、reactive brilliant yellow K-6G are 94.1%、96.9%、94.3%、50.7% respectively.Through further study on its morphological character, growth characteristics and enzyme production,we found that CFCC-4 of Coriolus versicolor contains few spores and hypha takes up the main thalli structure.Hypha twist together irregularly. CFCC-4 of Coriolus versicolor is a kind of white rot fungi mainly producing MnP and Lac.When cultured under shaking and N-S condition,its biomass reaches the maximum on the eighth day.On the ninth day,the enzyme activity of MnP reaches the maximum,2819 U/L and on the twelfth day, the enzyme activity of Lac reaches the maximum,2897U/L.(2) Under non-sterilized condition,culture CFCC-4 of Coriolus versicolor and optimize decolorization technological parameters.Four kinds of reactive dye were selected as the type dyes for the research on decolorization degradation:reactive brilliant red X-3B, λmax=513nm; reactive brilliant red KD-8B, λmax=545nm; reactive brilliant blue KN-R, λmax=568nm; reactive brilliant yellow K-6G, λmax=410nm.The experiment result is as follows:towardly fermentation condition can improve the decolorization effect on reactive dyes by CFCC-4 of Coriolus versicolor.Regardless of non-sterilized condition or sterilization condition,when the carbon source is sucrose,the nitrogen source is ammonium tartrate,the concentration of sucrose is 20g/L,the concentration of ammonium tartrate is 2.2g/L and the temperature is 28℃,it has the best discolorization effect on reactive dyes.After 144 hours,the decolorization rates of four kinds of reactive dyes by CFCC-4 of Coriolus versicolor have improved in certain degree.The decolorization rate:reactive brilliant red X-3B 96.4%, reactive brilliant red KD-8B 98.2%, reactive brilliant blue KN-R 95.9%, reactive brilliant yellow K-6G 69.5%.Compared with the the decolorization rate before optimization of 94.1%、96.9%、90.5% and 50.7%,the rate respectively improves by 5.3%、1.3%、5.4% and 18.8%.(3) Through contrasting sterilization condition and non-sterilized condition,different culture conditions,pH value,dye concentrations, thalli culture time and thalli biomass,we found that when pH value is 4.5 and thalli culture time is 9 days,adding thalli(three pieces per 90mL/250mL) into reactive dye with the concentration of 60mg/L and culturing thalli under oscillating condition can make the highest decolorization rate by CFCC-4 of Coriolus versicolor under non-sterilized condition.(4) Known from continuous intermittent culture,CFCC-4 of Coriolus versicolor can degrade reactive brilliant red X-3B, reactive brilliant red KD-8B and reactive brilliant blue KN-R for successive four generations and the decolorization rate can approach 100% in the end,but it can’t degrade reactive brilliant yellow K-6G successively and can degrade only a batch of dye waste water at most.The decolorization rate is not high and the decolorization effect isn’t good.(5) Prepare compounded dyes waste water with different proportions and research the degradation capability of the compounded dyes. When the concentration of compounded reactive dyes waste water is 50-250mg/L,CFCC-4 of Coriolus versicolor can decolorize it efficiently under sterilization condition and non-sterilized condition as well.After 60 hours,the decolorization rate is more than 80%.Besides,under non-sterilized condition,when the concentration of the compounded dyes waste water is 200mg/L,the decolorization effect is the best.Under sterilization condition,when the concentration of the compounded dyes waste water is 100mg/L,the decolorization effect is the best.