The Separation And Identification Of Camellia Nitidissima Chi And Their Potential Activity Evaluation

Objective:Camellia nitidissima chi, a genus of flowering plants in the family Theaceae, belongs to deciduous leaf bush, and have been traditionally used in China as healthy food and Chinese medicines. The yellow petals of the flowers are rarely found in the world, so it is called "The plant kingdom of giant pandas". To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima Chi. The structures of these compounds were identified on the basis of spectral data including NMR and MS. Then quorum sensing inhibition (QSI) activities of these compounds and the antitumor activities of these compounds were measured.Method:The chemical constituents (methylene chloride and ethyl acetate parts of Camellia nitidissima chi) were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel, recrystallization and semi-preparative HPLC techniques. The structures of these compounds were identified on the basis of spectral data including NMR and MS. Then quorum sensing inhibition (QSI) activities of these compounds were tested by QSI modifications, The activities of these compounds were measured by CCK-8 as cell proliferation and cytotoxicity. The antitumor activities of these compounds were measured by MTT.Results:(1) a-spinasteryl-β-D-glucopyranoside (2) stigmasta-7,22-diene- 3-O-[α-L-arabinopyranosyl (1â†'2)]-β-D-galactopyranoside (3) kaempferol 3-O-[2-O-(trans-p-coumaroyl)-3-O-a-D-glucopyranosyl]-a-D-glucopyranoside (4) aromadendrin (5) catechin (6) phlorizin 4’-O-β-D-glucopyranoside (7) (3R,6R,7E)-3-hydroxy-4,7-megastigmadien-9-one (8) dodecanoic acid (9) 3β-acetoxy-20-lupanol (10) 3β,6α,13β-trihydroxyolean-7-one (11) barrigenol A1 22-angelate (12) 3β,6a,13β-trihydroxyolean-7-one (13) rubiprasin (14) β-D-Galactopyranoside,(2S)-2-(acetyloxy)-3-[[(9Z)-1-oxo-9-octadecen-1-yl]oxy]propyl (15) β-D-Glucopyranoside,3-[(1-oxo-9,12,15-octadecadienyl)oxy]-2-[(1-oxo-9,12,15- octadecatr-ienyl)oxy] (16) P-D-Glucopyranoside,3-[(1-oxo-9,12-octadecadienyl)oxy]-2-[(1-oxo-9,12,15-octadecatrienyl)oxy]propyl (17) β-D-Glucopyranoside,2-[[(9Z,12Z,15Z)-1-oxo- 9,12, 15-octadecatrien-1-yl]oxy]-3-[(1-oxooctyl)oxy]propy (18) β-sitosterol were successively isolated from the leaves of Camellia nitidissima Chi. Apart from compound (4) (5) and (8), other 15 compounds are reported in this plant for the first time. All compounds showed no QSI activity, compound (10) showed potential cytotoxic activity on SGC7901 cells in vitro (IC5o=91.7 μg/mL), and compound (11) showed the activity of inhibiting tumor A549, HGC-27, MDA-MB-435 and SW620.