Study On Extraction, Purification And Properties Of Pigment From Lonicera Edulis Anthocyanins

In recent years, with the development of human health, natural pigments are widely used in food. Lonicera edulis contains large amounts of anthocyanins, and rich nutrition, unique flavor, with potential medicinal value and commercial value. Therefore, the prospect of Lonicera edulis resources development and use is very wide.In this study, Lonicera edulis fresh fruit was as the material, research the extraction, depuration, stabilization, resistance to oxidation of anthocyanins, in hope to provide the basis for the development of novel natural pigment.Compare the effect of solvent extraction and ultrasonic saasisted extraction by two kinds of methods of Lonicera edulis anthocyanin. The optimal conditions of the solvent method was 60% ethanol, time was 100 min, liquid solid ration was 1:3, temperature was 55℃. The content of anthocyanin was 86.5mg/100 g and extraction rate for 78.30%. And the optimal condition of the ultrasonic assisted method was 55% ethanol, ultrasonic power was 55 W, time was 35 min, liquid solid was 1:3, temperature was 50℃. The content of anthocyanin was 103.7mg/100 g and extraction rate for 81.42%. Comparing the two methods, it can be known that the ultrasonic assisted extraction has higher extraction rate, shorter extraction time and higher content of anthocyanins than the solvent extraction method.Through the static adsorption test, the purification of 4 kinds of macroporous resins S-8, AB-8, D101, X-5 of Lonicera edulis anthocyanin was compared. The X-5 macroporous resin’s effect was the best. Through the dynamic adsorption test, the optimum conditions was obtained, sample volume flow 1mL/min, sample concentration 8mg/mL, maximum sample size 1700 mL, elution volume flow 1m L/min, maximum dosage of elution agent 120 mL. Under this condition, the color value of the Lonicera edulis anthocyanin after purification increased from 6.73 to 78.31, increased nearly 12 times.Stability experiment showed that Lonicera edulis anthocyanin should be stored in low temperature, acidity and dark conditions. Na+、K+、Ca2+、Mg2+、Mn2+ had no influences on its stability. Fe3+(<0.002mol/L) and Cu2+(<0.01mol/L) had hyperchromic effect, further concentration increase had inhibitory effect. Pb2+ and Zn2+ should avoid to contact. Lonicera edulis anthocyanin antioxidant ability and anti reduction ability was weak, should avoid to contact with H2O2 and Na2SO3. The glucose(>10%), cane sugar, tartaric acid, succinic acid and citric acid had hyperchromic effect. Potassium sorbate, sodium benzoate and sodium erythorbate had negative effects on the stability of the anthocyanin, and vitamin C had the hyperchromic effect. The test of scavemging free radicals and was discussed.When the concentration was 2mg/mL, Lonicera edulis anthocyanin on scavenging superoxide free radical rate was 75.95%,the IC50 was 0.865mg/mL. When the concentration was 0.2mg/mL, Lonicera edulis anthocyanin on scavenging DPPH free radical rate was 78.12%,the IC50 was 0.067mg/m L. When the concentration was 2mg/m L, Lonicera edulis anthocyanin on scavenging hydroxyl free radical rate was 87.94%,the IC50 was 0.408mg/mL.