Sepaeration And Reseach On Antioxidant And Anticancer Anthocyanin Of Lonecera Edulis

Lonicera edulis as a wild berries with rich biological active components, and has high nutrition and health care function. It’s anthocyanins are natural non-toxic, can be used as natural pigment in food pigment application. Lonicera edulis anthocyanin has antioxidant and anticancer effects, there are many reports, but mostly on anthocyanin bold or purified anthocyanins, Lonicera edulis anthocyanins in many types, different types of anthocyanin antioxidant and anticancer effects has not been reported yet.The experimental study of Lonicera edulis anthocyanin by seperation and purification, the comparison of antioxidant and anticancer effects, screening out the most powerful elution, and also did study of Lonicera edulis anthocyanin extraction, paving the way for a follow-up experiment. Designed for the future development and utilization of reference.Using organic solvent extraction of Lonicera edulis anthocyanin, through the single factor and response surface experiment to determine the optimal extraction conditions. The factors of Lonicera edulis anthocyanin extraction volume effect from big to small is:ethanol concentration> temperature> liquid ratio. Extraction of ethanol concentration is61%, liquid ratio is81:1, the extraction temperature is59degrees, extraction time is60min.The use of different free radical evaluation system comparison of anthocyanin extracts and purified the eluted analyte oxidation resistance, screening out the clearance rate highest fractions. The experimental results show that in the OH system,60%ethanol elution of anthocyanin eluate on hydroxyl radical scavenging rate is highest; in O2-·system,60%ethanol elution of anthocyanin eluate on superoxide radical scavenging rate is highest; in the DPPH system,40%ethanol elution patterns glycoside eluate on DPPH radical scavenging rate is highest; in the ABTS system,10%ethanol elution of anthocyanin eluate on ABTS radical scavenging rate. Overall,10%,40%,50%and60%ethanol elution of antioxidant activity higher than the20%,30%,70%and80%ethanol elution.Different Lonicera edulis anthocyanin elution effects on human lung cancer A-549, human hepatoma HepG2and human cervical carcinoma Hela three common tumor cells of24h,48h and72h, using four methyl thiazolyl tetrazolium salt (MTT) colorimetric method for the determination of cancer cell inhibition rate. The experimental results show that each kind of Lonicera edulis anthocyanin eluate on three kinds of tumor cells are inhibited, and presents the dose-effect and time-effect relationship. Among the10%ethanol elution of anthocyanin eluate on three kinds of tumor cell inhibition rate maximum.To sum up, Lonicera edulis has very good antioxidant and anticancer effects, it is developed and applied to food medicine and other fields of the natural green food.