Extraction,Purification And Antiglycation Of Polysaccharides From Lonicera Edulis

The Lonicera edulis polysaccharide was extracted,purified with Lonicera edulis as raw material,the molecular weight and monosaccharide composition were determined and its inhibitory effect on the in vitro glycosylation model was researched.The orthogonal test was used to optimize the extraction process through hot water extraction and complex enzyme extraction of Lonicera edulis polysaccharides.The optimal extraction conditions of hot water extraction showed that the ratio of solid to liquid,extraction time and extraction temperature was 1:20g/mL,3h and 50℃,respectively,the yield of polysaccharides was 21.53%.The optimum conditions of compound enzyme extraction showed that,the amount of compound enzyme,enzymolysis temperature and enzymolysis time was 4%(Pectinase 960 U/g and Cellulase 800 U/g),60℃ and 45min,respectively,the yield of polysaccharides was 24.43%under these conditions.The response surface method was used to optimize the polysaccharides extraction processes of Lonicera edulis with ultrasonic-assisted.The optimum conditions as follows,ultrasonic time,extraction temperature and ultrasonic power was 67min,41℃ and 175W,respectively,the result was 24.324%under the conditions of validation experiments.The ultrasonic-assisted method of the extraction of Lonicera edulis was the best method.The crude polysaccharides was decolorized by activated carbon.The amount of activated carbon added,the decolorization temperature and the decolorization time was 4%,60℃and 60min,respectively,the decolorization rate was 98.1%and the polysaccharide retention rate was 95%under these conditions.Separation and purification of Lonicera edulis polysaccharide with DEAE-Sepharose Fast Flow ion exchange column chromatography and Sephadex G-100 dextran gel column chromatography,a single symmetrical peak was obtained which indicated that Lonicera edulis polysaccharide was a single component after separating,the recovery rate was 59.67%.The molecular weight of Lonicera edulis polysaccharide was 8375Da with HPLC determined,Gas chromatography(GC)was used to determine the monosaccharide composition,the result show that composition of Lonicera edulis polysaccharide is glucose.The model system of BSA-glucose was established in vitro.Lonicera edulis polysaccharide as natural inhibitor.Aminoguanidine as a positive control.Lonicera edulis polysaccharide were used to inhibit fructosamine,glyoxal(GO),5-hydroxymethylfurfural(5-HMF),Pentosidine and AGEs.It was confirmed that the Lonicera edulis polysaccharide had a certain effect in preventing the cross-linking of sugars and proteins by SDS-PAGE.Different concentrations of Lonicera edulis polysaccharide have a certain inhibitory effect on AR.